Tumor Etiology and Screening Department of Cancer Institute and General Surgery, The First Affiliated Hospital of China Medical University, Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, 155# North Nanjing Street, Heping District, Shenyang City, 110001, Liaoning Province, China.
Dig Dis Sci. 2018 Nov;63(11):2959-2968. doi: 10.1007/s10620-018-5224-z. Epub 2018 Jul 27.
Damage-specific DNA binding protein 2 (DDB2) is implicated in the recognition of DNA damage and the initiation of nucleotide excision repair process. The aim of this study was to explore the role of DDB2 in the initiation, progression, and prognosis of colorectal cancer (CRC).
Totally tissues of 300 CRC and 300 adjacent, 267 colorectal adenoma (CRA) and 214 normal (NOR) were collected. The expression of DDB2 protein was detected by immunohistochemical staining.
DDB2 protein was highly expressed in CRC and CRA compared with NOR (P < 0.001, respectively) in the dynamic sequence of NOR → CRA → CRC; CRC tissue demonstrated increased DDB2 expression compared with non-tumor adjacent tissues (P < 0.001). DDB2 expression was higher in T1-T2 than that in T3-T4 in CRC (P = 0.023); cloddy/nested CRC demonstrated increased DDB2 expression than infiltrative CRC (P = 0.007). Survival analysis showed that high DDB2 expression was associated with favorable survival in colon cancer (adjusted HR 0.20, 95% CI 0.06-0.72, P = 0.014) and female CRC patients (adjusted HR 0.27, 95% CI 0.08-0.92, P = 0.036).
DDB2 protein expression was associated with the initiation, progression, and prognosis of CRC, and might function as a tumor biomarker for the diagnosis and prognosis of CRC.
损伤特异性 DNA 结合蛋白 2（DDB2）参与 DNA 损伤的识别和核苷酸切除修复过程的启动。本研究旨在探讨 DDB2 在结直肠癌（CRC）的发生、发展和预后中的作用。
收集 300 例 CRC 及 300 例癌旁组织、267 例结直肠腺瘤（CRA）和 214 例正常组织（NOR）。采用免疫组化法检测 DDB2 蛋白的表达。
DDB2 蛋白在 CRC 和 CRA 中的表达均高于 NOR（分别为 P<0.001），呈 NOR→CRA→CRC 的动态序列；CRC 组织中 DDB2 蛋白的表达高于非肿瘤旁组织（P<0.001）。CRC 中 T1-T2 期 DDB2 蛋白的表达高于 T3-T4 期（P=0.023）；巢状/浸润性 CRC 中 DDB2 蛋白的表达高于弥漫性 CRC（P=0.007）。生存分析显示，DDB2 高表达与结肠癌（调整 HR 0.20，95%CI 0.06-0.72，P=0.014）和女性 CRC 患者（调整 HR 0.27，95%CI 0.08-0.92，P=0.036）的生存相关。
DDB2 蛋白的表达与 CRC 的发生、发展和预后相关，可能作为 CRC 诊断和预后的肿瘤标志物。
