Bayram Dilek, Armagan İlkay, Özgöcmen Meltem, Senol Nurgül, Calapoglu Mustafa
Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey.
Department of Nutrition and Dietetics, Faculty of Health Sciences, Süleyman Demirel University, Isparta, Turkey.
J Environ Pathol Toxicol Oncol. 2018;37(2):173-181. doi: 10.1615/JEnvironPatholToxicolOncol.2018025226.
This study aimed to investigate the effects of juglone on the human bladder carcinoma cell lines TCC-SUB and RT-4 in monolayer and spheroid cultures. Cells were treated with juglone at 24, 48, and 72 h of incubation. The activity of caspase-3 was detected in vitro using a caspase-3 colorimetric assay kit according to the manufacturer's instructions. The bromodeoxyuridine (BrdU) labeling index was used to determine the cells of the synthesis phase. The terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay was used to determine the death of cells in both the monolayer and spheroid cultures. The control group had a large S-phase fraction and many of the TCC-SUB and RT-4 cells nuclei were observed to be positive for BrdU. The dead cell count was higher in the TCC-SUB and RT-4 cell lines with juglone applied than in the controls. We conclude that juglone significantly inhibits the proliferation and induces the apoptosis of TCC-SUB and RT-4 cells in vitro.
本研究旨在探讨胡桃醌对人膀胱癌细胞系TCC - SUB和RT - 4单层培养及球状体培养的影响。在培养24、48和72小时时用胡桃醌处理细胞。根据制造商的说明，使用半胱天冬酶 - 3比色测定试剂盒在体外检测半胱天冬酶 - 3的活性。用溴脱氧尿苷（BrdU）标记指数来确定处于合成期的细胞。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法来确定单层培养和球状体培养中细胞的死亡情况。对照组有较大的S期比例，并且观察到许多TCC - SUB和RT - 4细胞核的BrdU呈阳性。应用胡桃醌的TCC - SUB和RT - 4细胞系中的死细胞计数高于对照组。我们得出结论，胡桃醌在体外显著抑制TCC - SUB和RT - 4细胞的增殖并诱导其凋亡。
