Bayram Dilek, Özgöçmen Meltem, Armagan Ilkay, Sevimli Murat, Türel Gülçin Yavuz, Şenol Nurgül
Department of Histology and Embryology Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey.
Department of Medical Biology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey.
J Cancer Res Ther. 2019 Jan-Mar;15(1):68-74. doi: 10.4103/jcrt.JCRT_880_17.
Colon cancer is a major cause of morbidity and mortality in the world. Juglone is a natural compound which has been isolated from Juglans mandshurica Maxim, and it has various pharmacological effects such as antiviral, antibacterial, and anticancer. In our study, we aimed to investigate the effect of juglone on CCL-228-SW 480 colon carcinoma cell line in monolayer and spheroid culture medium.
The CCL-228-SW 480 cell lines were cultured in both monolayer and spheroid cultures. Cells were treated with juglone at 24, 48, and 72 h of incubation. ID50 inhibition was determined on the dose for juglone and after it was found 20 μM was applied to the cells to examine the effect of juglone on CCL-228-SW 480 colon carcinoma cell line. After Juglone was applied the BrdU marking index, Transferase dUTP Nick ends Labeling (TUNEL) assay, active caspase-3 assay, apoptosis-inducing factor (AIF) assay were determined by immunohistochemistry in both the monolayer and spheroid cultures.
The control group had a healthy pattern of S-phase fraction, and many of the CCL-228-SW 480 cells nuclei were observed to be positive for BrdU. Terminal Deoxynucleotidyl TUNEL-positive cells, active Caspase-3, and AIF were detected after treatment with juglone in both the monolayer and spheroid cultures.
The dead cell count was higher in the CCL-228-SW 480 cell lines with juglone applied than in the controls. Juglone significantly inhibits the proliferation and induces the apoptosis of CCL-228-SW 480 cells in vitro.
结肠癌是全球发病和死亡的主要原因。胡桃醌是一种从胡桃楸中分离出的天然化合物，具有抗病毒、抗菌和抗癌等多种药理作用。在本研究中，我们旨在研究胡桃醌对单层和球体培养基中CCL - 228 - SW 480结肠癌细胞系的影响。
CCL - 228 - SW 480细胞系在单层和球体培养中培养。在培养24、48和72小时时用胡桃醌处理细胞。确定胡桃醌的半数抑制剂量（ID50），发现20μM后将其应用于细胞，以研究胡桃醌对CCL - 228 - SW 480结肠癌细胞系的影响。应用胡桃醌后，通过免疫组织化学在单层和球体培养中测定BrdU标记指数、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）检测、活性半胱天冬酶 - 3检测、凋亡诱导因子（AIF）检测。
对照组具有健康的S期分数模式，观察到许多CCL - 228 - SW 480细胞核BrdU呈阳性。在单层和球体培养中用胡桃醌处理后均检测到末端脱氧核苷酸TUNEL阳性细胞、活性半胱天冬酶 - 3和AIF。
应用胡桃醌的CCL - 228 - SW 480细胞系中的死细胞计数高于对照组。胡桃醌在体外显著抑制CCL - 228 - SW 480细胞的增殖并诱导其凋亡。
