Younis Tahira, Rasul Azhar, Jabeen Faiza, Hussain Ghulam, Altaf Javaria, Jafri Laila, Rani Rehana, Khan Muhammad Rashid, Sarfraz Iqra, Ali Muhammad
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan / Depaertment of Zoology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.
Depaertment of Zoology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.
Pak J Pharm Sci. 2018 Jul;31(4(Supplementary)):1475-1484.
Roots, bark, stem/twigs, and leaves of Fraxinus xanthoxyloides are being used regionally for the cure of malaria, jaundice, internal injuries, pneumonia, pain, rheumatism and also in fracture of bones. Our objective was to assess the methanolic leaves extract of F. xanthoxyloides for its antioxidant capability against oxidative stress induced by carbon tetrachloride (CCl) in the kidney of Sprague-Dawley rats. Duration of this experiment was 30 days and doses were given on alternative days. Urine of rats was assessed for kidney function and renal tissues for antioxidant enzymes activity, biochemical markers, comet assay and histopathology. Enhanced urinary creatinine, urobilinogen levels and decreased creatinine clearance, protein contents, and albumin levels were observed by CCl administration when matched to controls. CCl injection also decreased the level of reduced glutathione, catalase, super oxide dismutase, peroxidase, glutathione s-transferase, glutathione reductase, and tissue protein while elevated the levels of thiobarbituric acid reactive substances, DNA damages and HO in renal tissues of experimental animals. Co-treatment of FXM and silymarin, lead to the restoration of all the above tested parameters of kidney. Through this study we affirmed the ameliorating role of F. xanthoxyloides in oxidative stress affiliated disorders of kidney.
在当地，清香木犀的根、树皮、茎/嫩枝和叶被用于治疗疟疾、黄疸、内伤、肺炎、疼痛、风湿，也用于治疗骨折。我们的目的是评估清香木犀叶的甲醇提取物对四氯化碳（CCl）诱导的Sprague-Dawley大鼠肾脏氧化应激的抗氧化能力。本实验为期30天，每隔一天给药一次。对大鼠尿液进行肾功能评估，对肾组织进行抗氧化酶活性、生化标志物、彗星试验和组织病理学评估。与对照组相比，给予CCl后，大鼠尿肌酐、尿胆原水平升高，肌酐清除率、蛋白质含量和白蛋白水平降低。CCl注射还降低了实验动物肾组织中还原型谷胱甘肽、过氧化氢酶、超氧化物歧化酶、过氧化物酶、谷胱甘肽S-转移酶、谷胱甘肽还原酶和组织蛋白的水平，同时提高了硫代巴比妥酸反应性物质、DNA损伤和HO的水平。FXM和水飞蓟宾联合治疗可使上述所有肾脏测试参数恢复正常。通过本研究，我们证实了清香木犀在肾脏氧化应激相关疾病中的改善作用。
