Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, P. R. China.
Savaid Medical School, University of the Chinese Academy of Sciences, Beijing, P. R. China.
J Sep Sci. 2018 Oct;41(19):3660-3668. doi: 10.1002/jssc.201800411. Epub 2018 Aug 8.
Six compounds including two n-butyrophenone isomers and two stibene isomers were obtained from Rheum tanguticum Maxim. Two n-butyrophenone isomers with a separation factor of 1.14 were successfully separated by recycling high-speed counter-current chromatography after ten cycles. Two stibene isomers were successfully separated by preparative high-performance liquid chromatography. High-performance liquid chromatography analysis showed that the purities of the compounds were all over 98%. These compounds were identified as lindleyin, isolindleyin, resveratrol-4'-O-(2″-O-galloyl)-glucopyranoside, resveratrol-4'-O-(6''-O-galloyl)-glucopyranoside, emodin 1-O-β-d-glucoside, and 3,5-dihydroxy-4'-methoxystilbene-3-O-β-d-glucopyranoside. The results indicated that recycling high-speed counter-current chromatography and preparative high-performance liquid chromatography could be effective combination for the preparation of bioactive compounds from Rheum tanguticum Maxim.
从大黄中分离得到 6 种化合物，包括 2 种丁基苯酮异构体和 2 种二苯乙烯异构体。经过 10 次循环，通过高速逆流色谱成功分离出两种分离因子为 1.14 的丁基苯酮异构体。通过制备高效液相色谱成功分离出两种二苯乙烯异构体。高效液相色谱分析表明，化合物的纯度均在 98%以上。这些化合物分别被鉴定为大黄酸、异大黄酸、白藜芦醇-4'-O-(2″-O-没食子酰基)-吡喃葡萄糖苷、白藜芦醇-4'-O-(6''-O-没食子酰基)-吡喃葡萄糖苷、大黄素 1-O-β-D-吡喃葡萄糖苷和 3,5-二羟基-4'-甲氧基二苯乙烯-3-O-β-D-吡喃葡萄糖苷。结果表明，高速逆流色谱的循环使用和制备高效液相色谱可以有效地结合起来，从大黄中制备生物活性化合物。
