Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, UP, India.
IgY Immunologix Pvt. Ltd. H.No. 3-14/2, Survey No. 312, Narsingi Village, Rajendranagar Mandal, Rangareddy District, Hyderabad, 500075, India.
Sci Rep. 2018 Jul 30;8(1):11441. doi: 10.1038/s41598-018-29490-8.
Only three immediate early genes (IE) BICP0, BICP4 and BICP22 of Bovine herpesvirus 1 (BoHV-1) are known. These genes are expressed coordinately and their promoters are well characterized. We provide evidence for expression of three additional IE genes of BoHV-1 i.e. UL21, UL33 and UL34. These genes are expressed in the presence of cycloheximide (CH) at the same time as known IE genes. Surprisingly, the promoters of newly identified IE genes (UL21, UL33, UL34) lack the OCT-1 binding site, a considered site of transactivation of the BoHV-1 IE genes. The other difference in the promoters of the newly identified IE genes is the presence of TATA box at near optimal site. However, all the IE genes have similar spatial placements of C/EBPα, DPE and INR elements.
仅有三种牛疱疹病毒 1（BoHV-1）的即刻早期基因（IE），分别为 BICP0、BCP4 和 BICP22。这些基因协调表达，其启动子也已得到很好的描述。我们提供了牛疱疹病毒 1 另外三种即刻早期基因（UL21、UL33 和 UL34）表达的证据。在存在环己酰亚胺（CH）的情况下，这些基因与已知的即刻早期基因同时表达。令人惊讶的是，新鉴定的即刻早期基因（UL21、UL33、UL34）的启动子缺乏 OCT-1 结合位点，该位点被认为是 BoHV-1 IE 基因的转录激活位点。新鉴定的即刻早期基因启动子的另一个差异是存在接近最佳位置的 TATA 盒。然而，所有的即刻早期基因都具有相似的 C/EBPα、DPE 和 INR 元件的空间排列。
