The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Midlothian EH25 9RG, UK.
Viruses. 2024 Sep 30;16(10):1549. doi: 10.3390/v16101549.
Bovine herpesvirus type 1 (BoHV-1) causes severe diseases in bovine species and great economic burden to the cattle industry worldwide. Due to its complex life cycle, many host factors that affect BoHV-1 replication remain to be explored. To understand the possible roles that the Oct1 cellular protein could play in this process, we first created Oct1-deficient MDBK cells using CRISPR/Cas9-mediated genome editing. Upon infection, the absence of Oct1 in MDBK cells significantly impacted BoHV-1 replication, a phenotype rescued by over-expressing the wild-type Oct1 protein in the deficient cells. We further found that the expression of all three classes of temporal genes, including essential and non-essential viral genes, were significantly reduced in Oct1 knockout MDBK cells, following both high and low multiplicity of infection. In summary, our findings confirm that the bovine Oct1 protein acts as a pro-viral factor for BoHV-1 replication by promoting its viral gene transcription in MDBK cells.
牛疱疹病毒 1 型（BoHV-1）可引起牛科动物的严重疾病，给全球养牛业带来巨大的经济负担。由于其复杂的生命周期，许多影响 BoHV-1 复制的宿主因素仍有待探索。为了了解 Oct1 细胞蛋白在这一过程中可能发挥的作用，我们首先使用 CRISPR/Cas9 介导的基因组编辑技术创建了 Oct1 缺陷型 MDBK 细胞。感染后，MDBK 细胞中 Oct1 的缺失显著影响了 BoHV-1 的复制，而在缺陷细胞中过表达野生型 Oct1 蛋白可挽救这种表型。我们还发现，在高和低感染复数下，Oct1 敲除 MDBK 细胞中所有三类瞬时基因（包括必需和非必需病毒基因）的表达均显著降低。总之，我们的研究结果证实，牛 Oct1 蛋白在 MDBK 细胞中通过促进其病毒基因转录，作为 BoHV-1 复制的促病毒因子发挥作用。
