Moulavi F, Hosseini S M
Department of Embryology, Camel Advanced Reproductive Technologies Centre, P.O.Box: 7777, Dubai, United Arab Emirates.
Department of Embryology, Camel Advanced Reproductive Technologies Centre, P.O.Box: 7777, Dubai, United Arab Emirates.
Theriogenology. 2018 Oct 1;119:259-267. doi: 10.1016/j.theriogenology.2018.07.010. Epub 2018 Jul 18.
During in vitro maturation (IVM), the degree and pattern of cumulus cells expansion vary between cumulus oocyte complexes (COCs). This study investigated the relationship between expansion morphology of cumulus cells with the oocyte maturation quality in dromedary camel. Following IVM for 30-32 h, COCs were classified into four distinct groups according to the morphological features of COCs (adherent vs. non-adherent to the bottom of culture dish) and cumulus cells (compact vs. expanded vs. dissociated). The predominant morphology was adherent/dissociated (45.6 ± 7.1%), followed by adherent/compact (31.2 ± 6.5%), and then non-adherent/expanded (13.1 ± 2.8%) and non-adherent/compact (10.2 ± 2.4%). The adherent/dissociated morphology was correlated with the best oocyte quality in terms of MII-maturation (90.6 ± 5.0%), degeneration (22.4 ± 5.3%), reactive oxygen species (2.5 ± 0.5 arbitrary units), mitochondrial potential (12.9 ± 2.4 orange/green fluorescence intensity ratio), zona dissolution time (46 s), peripheral distribution of cortical granules (92%), and also cleavage and blastocyst development (81.3 ± 5.2 and 48.7 ± 7.2%, respectively). In contrast, adherent/compact morphology was correlated with the lowest oocyte competence when examined for the aforementioned criteria (28.1 ± 5.3%, 62.7 ± 9.2%, 6.0 ± 0.8, 2.5 ± 1.3, 102 s, 36%, and 25.7 ± 4.5 and 11.6 ± 5.1%, respectively). Non-adherent COCs, either expanded or compact, were correlated with an intermediate oocyte competence compared to the two extreme groups. In short, diverse pattern of cumulus expansion reveal heterogeneous cellular and molecular features associated with in vitro maturation capacity in camel. Cumulus expansion morphology can be used as a non-invasive predictive marker of oocyte competence to optimize assisted reproductive technologies in camels.
在体外成熟（IVM）过程中，卵丘细胞的扩展程度和模式在卵丘-卵母细胞复合体（COCs）之间存在差异。本研究调查了单峰驼中卵丘细胞扩展形态与卵母细胞成熟质量之间的关系。在IVM 30 - 32小时后，根据COCs的形态特征（是否附着于培养皿底部）和卵丘细胞的形态（紧密型、扩展型、解离型）将COCs分为四个不同的组。主要形态是附着/解离型（45.6±7.1%），其次是附着/紧密型（31.2±6.5%），然后是非附着/扩展型（13.1±2.8%）和非附着/紧密型（10.2±2.4%）。就MII期成熟（90.6±5.0%）、退化（22.4±5.3%）、活性氧（2.5±0.5任意单位）、线粒体膜电位（12.9±2.4橙/绿荧光强度比值）、透明带溶解时间（46秒）、皮质颗粒的周边分布（92%）以及卵裂和囊胚发育（分别为81.3±5.2%和48.7±7.2%）而言，附着/解离形态与最佳卵母细胞质量相关。相比之下，当根据上述标准进行检测时，附着/紧密形态与最低的卵母细胞能力相关（分别为28.1±5.3%、62.7±9.2%、6.0±0.8、2.5±1.3、102秒、36%以及25.7±4.5%和11.6±5.1%）。与两个极端组相比，非附着的COCs，无论是扩展型还是紧密型，都与中等卵母细胞能力相关。简而言之，卵丘扩展的不同模式揭示了与骆驼体外成熟能力相关的异质细胞和分子特征。卵丘扩展形态可以用作卵母细胞能力的非侵入性预测标志物，以优化骆驼的辅助生殖技术。
