Joel D D, Chanana A D, Cottier H, Cronkite E P, Laissue J A
Cell Tissue Kinet. 1977 Jan;10(1):57-69.
Cortical thymocytes of young adult mice were labeled in situ with radioactive DNA precursors. As a result of cell emigration and cell death, total thymic radioactivity decreased within 8 days to 10% or less of that present on day 1. Accumulation of thymic migrants in peripheral lymphoid organs was estimated by computing the net thymus-derived radioactivity in these tissues. Thymic cell death was assessed by comparing values obtained with 125I-UdR to those acquired with 3H-TdR; The results indicate that cortical thymocytes migrate to the spleen, mesenteric lymph node, femurs and intestine; nevertheless, only a small fraction of the activity originally present in the thymus was recovered in these organs; the vast majority of newly formed cortical thymocytes apparently die after a relatively short life span. Exclusive of the fraction which dies in situ, evidence for thymocyte death is seen in bone marrow; however, most migrants appear to terminate in the intestine.
用放射性DNA前体对成年幼鼠的皮质胸腺细胞进行原位标记。由于细胞迁移和细胞死亡，胸腺总放射性在8天内降至第1天的10%或更低。通过计算这些组织中胸腺来源的净放射性来估计外周淋巴器官中胸腺迁移细胞的积累。通过比较用125I-UdR获得的值与用3H-TdR获得的值来评估胸腺细胞死亡；结果表明，皮质胸腺细胞迁移至脾脏、肠系膜淋巴结、股骨和肠道；然而，这些器官中仅回收了最初存在于胸腺中的一小部分活性；绝大多数新形成的皮质胸腺细胞显然在相对较短的寿命后死亡。除了原位死亡的部分，在骨髓中可见胸腺细胞死亡的证据；然而，大多数迁移细胞似乎在肠道中终止。
