Pabst R, Trepel F
Anat Rec. 1979 Oct;195(2):341-55. doi: 10.1002/ar.1091950208.
Lymphocyte production by mesenteric lymph nodes of normal young pigs was studied by intranodal injections of either tritiated thymidine or tritiated deoxycytidine as DNA precursors. One or two days after selective labeling of the mesenteric lymph nodes the relative and absolute number of lymphocytes derived from mesenteric lymph nodes were determined autoradiographically in the following organs: mesenteric, cervical and inguinal lymph nodes, spleen, thymus, bone marrow, Peyer's patches, tonsil, different regions of the gut, lung and liver. The overall cell production of mesenteric lymph nodes, as derived from the sum of all labeled cells one day after labeling, was estimated to be about 7 X 10(9) lymphocytes. Up to 40% of all newly formed lymphocytes had already left the lymph nodes within one day and were found in all organs studied. There was a preferential homing to the mucosa of the small intestine, but a considerable number migrated to the spleen and even to the thymus and bone marrow. In lymphoid organs all labeled cells were small and medium-sized lymphocytes one and two days after labeling. In cervical lymph nodes, spleen, tonsil and Peyer's patches the relative distribution to T and B cell areas was determined. There was an obvious preference of newly formed lymph node cells to home to T cell areas. The differences of labeling between thymidine or deoxycytidine were surprisingly low.
通过向正常幼猪肠系膜淋巴结内注射氚标记的胸腺嘧啶核苷或氚标记的脱氧胞苷作为DNA前体，研究了肠系膜淋巴结产生淋巴细胞的情况。在对肠系膜淋巴结进行选择性标记后的1天或2天，通过放射自显影法在以下器官中测定源自肠系膜淋巴结的淋巴细胞的相对数量和绝对数量：肠系膜、颈和腹股沟淋巴结、脾脏、胸腺、骨髓、派伊尔结、扁桃体、肠道不同区域、肺和肝脏。标记后1天，根据所有标记细胞的总数估算，肠系膜淋巴结的总体细胞产生量约为7×10⁹个淋巴细胞。在1天内，高达40%的新形成淋巴细胞已离开淋巴结，并在所有研究的器官中被发现。它们优先归巢至小肠黏膜，但也有相当数量迁移至脾脏，甚至胸腺和骨髓。在标记后的1天和2天，淋巴器官中的所有标记细胞均为中小型淋巴细胞。在颈淋巴结、脾脏、扁桃体和派伊尔结中，测定了T细胞区和B细胞区的相对分布。新形成的淋巴结细胞明显优先归巢至T细胞区。胸腺嘧啶核苷和脱氧胞苷之间的标记差异出奇地小。
