Sheng Kangliang, Shui ShanShan, Yan Ling, Liu Changhong, Zheng Lei
1School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009 China.
Research Laboratory of Agricultural Environment and Food Safety, Anhui Modern Agricultural Industry Technology System, Hefei, 230009 China.
J Food Sci Technol. 2018 Aug;55(8):3292-3302. doi: 10.1007/s13197-018-3264-1. Epub 2018 Jul 10.
Ultraviolet (UV) irradiation has been related to the extension shelf-life and maintenance of postharvest quality in fruits. However, the comparison of UV-B and UV-C treatment on the biosynthesis of phenolic compounds of grape remain unclear. This study provides a comparison on the mechanism of phenolic secondary metabolism at the same dose of 3.6 kJ m UV treatment. Total phenolic compounds, total flavonoid, total flavanol, and total anthocyanin content and antioxidant activities of grapes after UV-C treatments were higher than those of the control and UV-B treatment. Among the evaluated parameters of individual phenolic compounds, the content of -resveratrol showed the highest percentage increase after the UV application. The transcriptions of , , , , and were higher in grapes treated by UV-C than in those treated by UV-B. The , , and genes were more induced in UV-B treatment than in control group. The same applied dose of UV-B or UV-C irradiation have different impact on gene expression and phenolic metabolites synthesis. The UV-C irradiation stimulated a higher gene expression of the phenolic compounds biosynthesis and also induced a greater accumulation of these metabolites at the same applied dose.
紫外线（UV）照射与水果采后保质期的延长及品质维持有关。然而，UV-B和UV-C处理对葡萄酚类化合物生物合成的比较仍不明确。本研究对3.6 kJ·m紫外线处理相同剂量下酚类次生代谢机制进行了比较。UV-C处理后葡萄的总酚类化合物、总黄酮、总黄烷醇和总花青素含量及抗氧化活性均高于对照和UV-B处理。在单个酚类化合物的评估参数中，白藜芦醇含量在紫外线处理后增加百分比最高。UV-C处理的葡萄中、、、、和的转录水平高于UV-B处理的葡萄。、、和基因在UV-B处理中比在对照组中诱导程度更高。相同剂量的UV-B或UV-C照射对基因表达和酚类代谢物合成有不同影响。在相同施加剂量下，UV-C照射刺激了酚类化合物生物合成的更高基因表达，也诱导了这些代谢物的更大积累。
