Zhang Kekun, Li Wanping, Ju Yanlun, Wang Xianhang, Sun Xiangyu, Fang Yulin, Chen Keqin
College of Enology, Northwest A&F University, Yangling 712100, China.
Foods. 2021 Mar 16;10(3):625. doi: 10.3390/foods10030625.
In this study, ultraviolet-C (UV-C) was utilized to improve the quality of post-harvest grape berries, and the transcriptomic and metabolomic basis of this improvement was elucidated. Berries of the red grape variety 'Zicui' and the white variety 'Xiangfei' were chosen to evaluate the effect of short- and long-term UV-C irradiation. Post-harvest UV-C application promoted malondialdehyde (MDA) and proline accumulation, and reduced the soluble solid content in berries. Both the variety and duration of irradiation could modulate the transcriptomic and metabolomic responses of berries to UV-C. Compared with the control, the differentially expressed genes (DEGs) identified under UV-C treatment were enriched in pathways related to metabolite accumulation, hormone biosynthesis and signal transduction, and reactive oxygen species (ROS) homeostasis. Flavonoid biosynthesis and biosynthesis of other secondary metabolites were the shared pathways enriched with differential metabolites. After long-term UV-C irradiation, cis-resveratrol accumulated in the berries of the two varieties, while the differential chalcone, dihydroflavone, flavonoid, flavanol, and tannin components primarily accumulated in 'Xiangfei', and some flavonols and anthocyanins primarily accumulated in 'Zicui'. Based on an exhaustive survey, we made a summary for the effect of UV-C in regulating the quality development of post-harvest grape berries. The results of this study may help to elucidate the mechanism by which UV-C functions and support its efficient application.
在本研究中，利用紫外线-C（UV-C）来提高采后葡萄浆果的品质，并阐明了这种品质提升的转录组学和代谢组学基础。选用红葡萄品种‘紫脆’和白葡萄品种‘香妃’的浆果来评估短期和长期UV-C照射的效果。采后施用UV-C促进了丙二醛（MDA）和脯氨酸的积累，并降低了浆果中的可溶性固形物含量。照射的品种和时长均可调节浆果对UV-C的转录组学和代谢组学响应。与对照相比，在UV-C处理下鉴定出的差异表达基因（DEGs）在与代谢物积累、激素生物合成和信号转导以及活性氧（ROS）稳态相关的途径中富集。类黄酮生物合成和其他次生代谢物的生物合成是差异代谢物富集的共同途径。长期UV-C照射后，顺式白藜芦醇在两个品种的浆果中积累，而差异查耳酮、二氢黄酮、类黄酮、黄烷醇和单宁成分主要在‘香妃’中积累，一些黄酮醇和花青素主要在‘紫脆’中积累。基于详尽的研究，我们总结了UV-C对采后葡萄浆果品质发育的影响。本研究结果可能有助于阐明UV-C发挥作用的机制，并支持其有效应用。
