Fischer V, Mason R P
Chem Biol Interact. 1986 Feb;57(2):129-42. doi: 10.1016/0009-2797(86)90033-5.
The possible metabolic activation of nitrosonaphthols, suspected carcinogens, was investigated by electron spin resonance (ESR) spectroscopy. Free radicals were found to be the primary metabolites formed during both the reduction and oxidation of these compounds. Whereas the one-electron oxidation of nitrosonaphthols is enzymatic and catalyzed by the peroxidase prototype, horseradish peroxidase, their one-electron reduction by reducing cofactors such as NADH or NADPH was not enhanced by rat liver microsomal enzymes. The ESR spectra of the radicals found during the oxidation of nitrosonaphthols were analyzed and characterized as iminoxyl free radicals. The reduction pathway leads to nitroxide free radicals with unusually low nitrogen hyperfine constants.
通过电子自旋共振（ESR）光谱法研究了疑似致癌物亚硝基萘酚可能的代谢活化作用。发现自由基是这些化合物还原和氧化过程中形成的主要代谢产物。亚硝基萘酚的单电子氧化是酶促反应，由过氧化物酶原型辣根过氧化物酶催化，而它们通过诸如NADH或NADPH等还原辅因子进行的单电子还原并未被大鼠肝微粒体酶增强。分析了亚硝基萘酚氧化过程中发现的自由基的ESR光谱，并将其表征为异亚硝基自由基。还原途径导致具有异常低的氮超精细常数的硝基自由基。
