去铁胺（去铁敏）与超氧自由基。一种酶损伤性氮氧化物的形成。
Desferrioxamine (Desferal) and superoxide free radicals. Formation of an enzyme-damaging nitroxide.
作者信息
Davies M J, Donkor R, Dunster C A, Gee C A, Jonas S, Willson R L
机构信息
Department of Biochemistry, Brunel University, Uxbridge, Middx., U.K.
出版信息
Biochem J. 1987 Sep 15;246(3):725-9. doi: 10.1042/bj2460725.
Abstract
In neutral solutions, desferrioxamine (Desferal) can react with the superoxide free radical, O2.- (possibly through its protonated form HO2.), to form a relatively stable nitroxide free radical, which can have a half-life of approx. 10 min at room temperature. The formation of the radical can be largely prevented by the presence of superoxide dismutase. The radical reacts rapidly with cysteine, methionine, glutathione, vitamin C and a water-soluble derivative of vitamin E. It also reacts rapidly with alcohol dehydrogenase, causing a loss of enzyme activity. The implications of these findings for mechanistic free-radical biochemistry and iron-chelation therapy could be considerable.
摘要
在中性溶液中，去铁胺（Desferal）可与超氧自由基O₂⁻（可能通过其质子化形式HO₂⁻）反应，形成相对稳定的氮氧自由基，该自由基在室温下的半衰期约为10分钟。超氧化物歧化酶的存在可在很大程度上阻止该自由基的形成。该自由基能与半胱氨酸、蛋氨酸、谷胱甘肽、维生素C和维生素E的水溶性衍生物迅速反应。它还能与乙醇脱氢酶迅速反应，导致酶活性丧失。这些发现对自由基生物化学机制和铁螯合疗法的意义可能相当重大。
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