Bell Tisza A S, Sen-Kilic Emel, Felföldi Tamás, Vasas Gabor, Fields Matthew W, Peyton Brent M
Department of Chemical and Biological Engineering, Center for Biofilm Engineering, Montana State University, Bozeman, MT, 59717, USA.
Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA.
Antonie Van Leeuwenhoek. 2018 Dec;111(12):2425-2440. doi: 10.1007/s10482-018-1132-7. Epub 2018 Aug 1.
The Carpathian Basin is a lowland plain located mainly in Hungary. Due to the nature of the bedrock, alluvial deposits, and a bowl shape, many lakes and ponds of the area are characterized by high alkalinity. In this study, we characterized temporal changes in eukaryal and bacterial community dynamics with high throughput sequencing and relate the changes to environmental conditions in Lake Velence located in Fejér county, Hungary. The sampled Lake Velence microbial populations (algal and bacterial) were analyzed to identify potential correlations with other community members and environmental parameters at six timepoints over 6 weeks in the Spring of 2012. Correlations between community members suggest a positive relationship between certain algal and bacterial populations (e.g. Chlamydomondaceae with Actinobacteria and Acidobacteria), while other correlations allude to changes in these relationships over time. During the study, high nitrogen availability may have favored non-nitrogen fixing cyanobacteria, such as the toxin-producing Microcystis aeruginosa, and the eutrophic effect may have been exacerbated by high phosphorus availability as well as the high calcium and magnesium content of the Carpathian Basin bedrock, potentially fostering exopolymer production and cell aggregation. Cyanobacterial bloom formation could have a negative environmental impact on other community members and potentially affect overall water quality as well as recreational activities. To our knowledge, this is the first prediction for relationships between photoautotrophic eukaryotes and bacteria from an alkaline, Hungarian lake.
喀尔巴阡盆地是一个主要位于匈牙利的低地平原。由于基岩的性质、冲积沉积物以及碗状地形，该地区的许多湖泊和池塘具有高碱度的特征。在本研究中，我们利用高通量测序对真核生物和细菌群落动态的时间变化进行了表征，并将这些变化与位于匈牙利费耶尔州的韦伦采湖的环境条件相关联。对2012年春季6周内六个时间点采集的韦伦采湖微生物种群（藻类和细菌）进行了分析，以确定与其他群落成员和环境参数之间的潜在相关性。群落成员之间的相关性表明某些藻类和细菌种群之间存在正相关关系（例如衣藻科与放线菌和酸杆菌），而其他相关性暗示了这些关系随时间的变化。在研究过程中，高氮可用性可能有利于非固氮蓝细菌，如产毒素的铜绿微囊藻，富营养化效应可能因高磷可用性以及喀尔巴阡盆地基岩的高钙和镁含量而加剧，这可能促进了胞外聚合物的产生和细胞聚集。蓝藻水华的形成可能会对其他群落成员产生负面环境影响，并可能影响整体水质以及娱乐活动。据我们所知，这是对来自匈牙利碱性湖泊的光合自养真核生物与细菌之间关系的首次预测。
