State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, China.
School of Biology and Food Engineering, Changshu Institute of Technology, Changshu, 215500, China.
Virol Sin. 2018 Aug;33(4):335-344. doi: 10.1007/s12250-018-0045-y. Epub 2018 Aug 1.
Porcine reproductive and respiratory syndrome virus (PRRSV) causes substantial economic losses to the global pig industry. Alternative polyadenylation (APA) is a mechanism that diversifies gene expression, which is important for tumorigenesis, development, and cell differentiation. However, it is unclear whether APA plays a role in the course of PRRSV infection. To address this issue, in this study we carried out a whole-genome transcriptome analysis of PRRSV-infected Marc-145 African green monkey kidney cells and identified 185 APA switching genes and 393 differentially expressed genes (DEGs). Most of these genes were involved in cellular process, metabolism, and biological regulation, and there was some overlap between the two gene sets. DEGs were found to be more directly involved in the antiviral response than APA genes. These findings provide insight into the dynamics of host gene regulation during PRRSV infection and a basis for elucidating the pathogenesis of PRRSV.
猪繁殖与呼吸综合征病毒（PRRSV）给全球养猪业造成了巨大的经济损失。可变多聚腺苷酸化（APA）是一种多样化基因表达的机制，对于肿瘤发生、发育和细胞分化很重要。然而，APA 是否在 PRRSV 感染过程中发挥作用尚不清楚。为了解决这个问题，本研究对 PRRSV 感染的 Marc-145 非洲绿猴肾细胞进行了全基因组转录组分析，鉴定出 185 个 APA 切换基因和 393 个差异表达基因（DEGs）。这些基因大多参与细胞过程、代谢和生物调节，两个基因集之间存在一些重叠。发现 DEGs 比 APA 基因更直接参与抗病毒反应。这些发现为 PRRSV 感染期间宿主基因调控的动态提供了深入的了解，并为阐明 PRRSV 的发病机制奠定了基础。
