Niu Tianming, Fan Tianqi, Wang Yingjie, Gao Kuipeng, Zhao Jinhui, Wang Ruyu, Chen Xiaolei, Xing Junhong, Qiu Jingjing, Zou Boshi, Fan Shuhui, Zhang Shi, Wu Qiong, Yang Guilian, Wang Nan, Zeng Yan, Cao Xin, Jiang Yanlong, Wang Jianzhong, Huang Haibin, Yang Wentao, Shi Chunwei, Li Zhipeng, Wang Chunfeng
College of Veterinary Medicine, Jilin Agricultural University, Changchun 130012, China.
Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun 130000, China.
Int J Mol Sci. 2025 Mar 3;26(5):2257. doi: 10.3390/ijms26052257.
Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that can cause reproductive disorders in sows and affect the breathing of piglets, seriously endangering pig breeding worldwide. In this study, NC8 was used as the expression delivery vector of foreign proteins, and a single-chain antibody was designed based on an mAb-PN9cx3 sequence. Three recombinant strains of , namely, NC8/pSIP409-pgsA'-PN9cx3-scFV(E), NC8/pSIP409-pgsA'-PN9cx3-HC(E), and NC8/pSIP409-pgsA'-PN9cx3-LC(E), were successfully constructed. In an in vitro test, the viral load of each experimental group was significantly lower than that of the control group ( < 0.01). In the piglet challenge protection test, the percentage of CD3CD8T cells in the blood of piglets given complex lactic acid bacteria was significantly increased before and after the challenge ( < 0.01); the body temperature of piglets in this group was normal, the viral load of each organ was reduced, and the obvious pathological changes in each tissue were alleviated. At the same time, the abundance of Bacteroides, Fusobacterium, and other bacteria in the intestinal tracts of the piglets changed, affecting the metabolism of carbohydrates and amino acids and the differentiation of Th1 and Th2 cells. This experiment provides a feasible strategy and method for the design of a PRRSV vaccine.
猪繁殖与呼吸综合征（PRRS）是一种传染病，可导致母猪繁殖障碍并影响仔猪呼吸，严重危害全球养猪业。本研究以NC8作为外源蛋白的表达载体，基于单克隆抗体PN9cx3序列设计了单链抗体。成功构建了3种重组菌株，即NC8/pSIP409-pgsA'-PN9cx3-scFV(E)、NC8/pSIP409-pgsA'-PN9cx3-HC(E)和NC8/pSIP409-pgsA'-PN9cx3-LC(E)。体外试验中，各实验组病毒载量均显著低于对照组（P<0.01）。仔猪攻毒保护试验中，给予复合乳酸菌的仔猪攻毒前后血液中CD3+CD8+T细胞百分比显著升高（P<0.01）；该组仔猪体温正常，各器官病毒载量降低，各组织明显病理变化减轻。同时，仔猪肠道中拟杆菌属、梭杆菌属等细菌丰度改变，影响碳水化合物和氨基酸代谢以及Th1和Th2细胞分化。本实验为猪繁殖与呼吸综合征病毒疫苗设计提供了可行的策略和方法。
