Centre of Chemistry, University of Minho, Braga, Portugal.
Institute of Physical and Theoretical Chemistry, Technical University, Braunschweig, Germany.
FEBS Lett. 2018 Sep;592(18):3040-3053. doi: 10.1002/1873-3468.13211. Epub 2018 Aug 24.
The marginal stability of globular proteins in the cell is determined by the balance between excluded volume effect and soft interactions. Quinary interactions are a type of soft interactions involved in intracellular organisation and known to have stabilising or destabilising effects on globular proteins. Recent studies suggest that globular proteins have structural flexibility, exhibiting more than one functional state. Here, we propose that the quinary-induced destabilisation can be sufficient to produce functional partially unfolded states of globular proteins. The biological relevance of this mechanism is explored, involving intracellular phase separation and regulatory stress response mechanisms.
球形蛋白质在细胞中的边缘稳定性取决于排除体积效应和软相互作用之间的平衡。五元相互作用是一种涉及细胞内组织的软相互作用，已知对球形蛋白质具有稳定或不稳定的影响。最近的研究表明，球形蛋白质具有结构灵活性，表现出不止一种功能状态。在这里，我们提出五元诱导的去稳定化足以产生球形蛋白质的功能性部分去折叠状态。探讨了这种机制的生物学相关性，涉及细胞内相分离和调节应激反应机制。
