Fondazione Banca dei Tessuti di Treviso, Treviso, Italy.
Innovation, Development and Planning Department, Ca' Foncello Hospital, Treviso, Italy.
PLoS One. 2018 Aug 2;13(8):e0201792. doi: 10.1371/journal.pone.0201792. eCollection 2018.
Microbiological contamination of retrieved tissues is a critical aspect of allograft safety and tissue banks must continuously implement decontamination procedures to minimize tissue contamination. In this study we compared the decontamination efficacy of a new antibiotic cocktail (cocktail B: BASE medium with Gentamicin, Meropenem and Vancomycin) with the cocktail previously adopted at Treviso Tissue Bank Foundation (FBTV) (cocktail A: RPMI medium with Ceftazidime, Lincomycin, Polymyxin B and Vancomycin). Two decontamination steps were carried out, the first immediately after retrieval, the second after processing. The contamination rate was calculated before processing (Time 1) and cryopreservation (Time 2) for total tissues, musculoskeletal tissues and cardiovascular tissues, and the bacterial species involved were analyzed. Cocktail A was used to decontaminate 3548 tissues, of which 266 were cardiovascular and 3282 musculoskeletal tissues. For cocktail A, total tissue contamination was 18.6% at Time 1 and 0.9% at Time 2, with 15.7% contaminated musculoskeletal tissues at Time 1 and 0.4% at Time 2, respectively, while cardiovascular tissues were 50% contaminated at Time 1 and 6.4% at Time 2. Cocktail B was used to decontaminate 3634 tissues of which 318 were cardiovascular and 3316 musculoskeletal tissues. For cocktail B, total tissue contamination was 8.6% at Time 1 and 0.2% at Time 2, with 7.6% contaminated musculoskeletal tissues at Time 1 and 0.03% at Time 2, respectively. Contamination of cardiovascular tissues was 20.4% at Time 1 and 1.9% at Time 2. Intergroup and intragroup contamination rates decreased statistically significantly (p<0.05). Our results have shown that cocktail B was more effective than cocktail A in killing bacteria in both cardiovascular and musculoskeletal tissues during the two decontamination cycles.
回收组织的微生物污染是同种异体移植物安全性的一个关键方面，组织库必须不断实施去污程序，以最大程度地减少组织污染。在这项研究中，我们比较了一种新的抗生素鸡尾酒（鸡尾酒 B：含庆大霉素、美罗培南和万古霉素的 BASE 培养基）与特雷维索组织库基金会（FBTV）先前采用的鸡尾酒（鸡尾酒 A：含头孢他啶、林可霉素、多粘菌素 B 和万古霉素的 RPMI 培养基）的去污效果。进行了两个去污步骤，第一个步骤是在回收后立即进行，第二个步骤是在处理后进行。在处理前（Time 1）和冷冻保存前（Time 2）计算总组织、肌肉骨骼组织和心血管组织的污染率，并分析涉及的细菌种类。用鸡尾酒 A 处理了 3548 个组织，其中心血管组织 266 个，肌肉骨骼组织 3282 个。对于鸡尾酒 A，总组织污染率在 Time 1 为 18.6%，在 Time 2 为 0.9%，肌肉骨骼组织在 Time 1 为 15.7%，在 Time 2 为 0.4%，而心血管组织在 Time 1 为 50%，在 Time 2 为 6.4%。用鸡尾酒 B 处理了 3634 个组织，其中心血管组织 318 个，肌肉骨骼组织 3316 个。对于鸡尾酒 B，总组织污染率在 Time 1 为 8.6%，在 Time 2 为 0.2%，肌肉骨骼组织在 Time 1 为 7.6%，在 Time 2 为 0.03%。心血管组织的污染率在 Time 1 为 20.4%，在 Time 2 为 1.9%。组间和组内污染率均有统计学显著降低（p<0.05）。我们的结果表明，在两个去污循环中，鸡尾酒 B 比鸡尾酒 A 更有效地杀灭心血管组织和肌肉骨骼组织中的细菌。
