Fondazione Banca Dei Tessuti del Veneto Onlus, Treviso, Italy.
PolitoBIOMed Lab, Politecnico Di Torino, Turin, Italy.
Cell Tissue Bank. 2024 Jun;25(2):401-410. doi: 10.1007/s10561-023-10072-6. Epub 2023 Jan 25.
Pericardial patches are currently used as reconstructive material in cardiac surgery for surgical treatment of cardiac septal defects. Autologous pericardial patches, either treated with glutaraldehyde or not, can be used as an alternative to synthetic materials or xenograft in congenital septal defects repair. The availability of an allogenic decellularized pericardium could reduce complication during and after surgery and could be a valid alternative. Decellularization of allogenic tissues aims at reducing the immunogenic reaction that might trigger inflammation and tissue calcification over time. The ideal graft for congenital heart disease repair should be biocompatible, mechanically resistant, non-immunogenic, and should have the ability to growth with the patients. The aim of the present study is the evaluation of the efficacy of a new decellularization protocol of homologous pericardium, even after cryopreservation. The technique has proven to be suitable as a tissue bank procedure and highly successful in the removal of cells and nucleic acids content, but also in the preservation of collagen and biomechanical properties of the human pericardium.
目前，心包补片被广泛应用于心血管手术中，用于治疗心脏间隔缺损。未经戊二醛处理或经戊二醛处理的自体心包补片可作为合成材料或异种移植物的替代品，用于修复先天性间隔缺损。同种脱细胞心包的出现可以减少手术中和手术后的并发症，是一种有效的替代方法。同种组织脱细胞的目的是减少可能引发炎症和组织钙化的免疫原性反应。用于先天性心脏病修复的理想移植物应该具有生物相容性、机械强度高、无免疫原性，并能够随着患者的生长而生长。本研究的目的是评估一种新的同种心包脱细胞化方案的有效性，即使在冷冻保存后也是如此。该技术已被证明可作为组织库处理程序，非常成功地去除细胞和核酸含量，同时还能保持人的心包膜的胶原和生物力学特性。
