百合棒状杆菌原生质体的转染
Transfection of Corynebacterium lilium protoplasts.
作者信息
Yeh P, Oreglia J, Sicard A M
出版信息
J Gen Microbiol. 1985 Dec;131(12):3179-83. doi: 10.1099/00221287-131-12-3179.
PMID:3007654
Abstract
A protoplast transfection system has been developed for a lysine-producing bacterium, Corynebacterium lilium, using the DNA of phage CL31. Phage CL31 is lytic and specific to C. lilium and has a genome of approximately 48 kb. The transfection procedure involves a polyethylene-glycol-mediated introduction of the DNA into lysozyme-treated cells and has a maximum efficiency of 3 X 10(4) transfectants per microgram DNA.
摘要
已开发出一种用于产赖氨酸细菌百合棒杆菌的原生质体转染系统，该系统使用噬菌体CL31的DNA。噬菌体CL31具有溶菌性且对百合棒杆菌具有特异性，其基因组约为48 kb。转染过程包括通过聚乙二醇介导将DNA导入经溶菌酶处理的细胞，每微克DNA的最大转染效率为3×10⁴个转染子。
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