Cosby W M, Casas I A, Dobrogosz W J
Department of Microbiology, North Carolina State University, Raleigh 27695-7615.
Appl Environ Microbiol. 1988 Nov;54(11):2599-602. doi: 10.1128/aem.54.11.2599-2602.1988.
Protoplasts of L. plantarum were produced by mutanolysin-lysozyme digestion at 50 degrees C and regenerated at a frequency of 1.6 to 3.8%. The addition of Tween 80 to the growth medium increased the length of time required for protoplast formation. When transfected with bacteriophage B2 DNA, transfection efficiencies ranged from 25 to 230 PFU/microgram of DNA and from 2.2 X 10(-5) to 4.7 X 10(-4) PFU per recovered protoplast. Total transfectant yield was 3.7 X 10(2) to 3.4 X 10(3) per treatment. Transformations with plasmid DNA were unsuccessful.
植物乳杆菌原生质体通过在50℃下用变溶菌素 - 溶菌酶消化产生，再生频率为1.6%至3.8%。向生长培养基中添加吐温80会增加原生质体形成所需的时间。用噬菌体B2 DNA转染时，转染效率范围为每微克DNA 25至230噬菌斑形成单位，每个回收的原生质体为2.2×10(-5)至4.7×10(-4)噬菌斑形成单位。每次处理的总转染子产量为3.7×10(2)至3.4×10(3)。用质粒DNA进行转化未成功。
