Infection, Immunity & Inflammation, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK.
Infection, Immunity & Inflammation, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK; Scottish National Blood Transfusion Service, Aberdeen, UK.
Cell Immunol. 2018 Oct;332:58-76. doi: 10.1016/j.cellimm.2018.07.008. Epub 2018 Jul 19.
Macrophages are key in orchestrating immune responses to micro-environmental stimuli, sensed by a complex set of surface receptors. The human cell line THP-1 has a monocytic phenotype, including the ability to differentiate into macrophages, providing a tractable, standardised surrogate for human monocyte-derived macrophages. Here we assessed the expression of 49 surface markers including Fc, complement, C-type lectin and scavenger receptors; TIMs; Siglecs; and co-stimulatory molecules by flow cytometry on both THP-1 monocytes and macrophages and following macrophage activation with seven standard conditioning/polarizing stimuli. Of the 34 surface markers detected on macrophages, 18 altered expression levels on activation. From these, expression of 9 surface markers were consistently altered by all conditioning regimens, while 9 were specific to individual polarizing stimuli. This study provides a resource for the study of macrophages and highlights that macrophage polarization states share much in common and the differences do not easily fit a simple classification system.
巨噬细胞在协调对微环境刺激的免疫反应中起着关键作用，这些刺激由一整套复杂的表面受体感知。人单核细胞系 THP-1 具有单核细胞表型，包括分化为巨噬细胞的能力，为人类单核细胞衍生的巨噬细胞提供了一种易于处理的标准化替代物。在这里，我们通过流式细胞术评估了 49 种表面标记物的表达，包括 Fc、补体、C 型凝集素和清道夫受体；TIMs；Siglecs；以及在 THP-1 单核细胞和巨噬细胞上以及用七种标准条件/极化刺激物激活巨噬细胞后的共刺激分子。在巨噬细胞上检测到的 34 种表面标记物中，有 18 种在激活时改变了表达水平。在这些标记物中，有 9 种表面标记物的表达被所有条件处理方案一致改变，而 9 种则是特定于单个极化刺激物的。本研究为巨噬细胞的研究提供了一个资源，并强调了巨噬细胞极化状态有很多共同点，并且差异不容易符合简单的分类系统。
