Higuchi Hiroshi, Shoji Toru, Iijima Shinji, Nishijima Ken-Ichi
a Department of Biotechnology , Nagoya University , Nagoya , Japan.
Biosci Biotechnol Biochem. 2016 Jun;80(6):1141-8. doi: 10.1080/09168451.2016.1146070. Epub 2016 Feb 29.
Siglecs recognize the sialic acid moiety and regulate various immune responses. In the present study, we compared the expression levels of Siglecs in human monocytes and macrophages using a quantitative real-time reverse transcription-polymerase chain reaction analysis. The differentiation of monocytes into macrophages by macrophage colony-stimulating factor or granulocyte macrophage colony-stimulating factor enhanced the expression of Siglec-7 and Siglec-9. The differentiated macrophages were stimulated by lipopolysaccharide (LPS) plus interferon (IFN)-γ or interleukin (IL)-4. The expression of Siglec-10 was enhanced by IL-4, whereas that of Siglec-7 was reduced by LPS plus IFN-γ. The expression of Siglec-9 was not affected by these stimuli. The knockdown of Siglec-9 enhanced the expression of CCR7 induced by the LPS or the LPS plus IFN-γ stimulation, and decreased the IL-4-induced expression of CD200R. These results suggest that Siglec-9 is one of the main Siglecs in human blood monocytes/macrophages and modulates innate immunity.
唾液酸结合免疫球蛋白样凝集素（Siglecs）可识别唾液酸部分并调节多种免疫反应。在本研究中，我们使用定量实时逆转录-聚合酶链反应分析比较了人单核细胞和巨噬细胞中Siglecs的表达水平。巨噬细胞集落刺激因子或粒细胞巨噬细胞集落刺激因子将单核细胞分化为巨噬细胞可增强唾液酸结合免疫球蛋白样凝集素-7（Siglec-7）和唾液酸结合免疫球蛋白样凝集素-9（Siglec-9）的表达。用脂多糖（LPS）加干扰素（IFN）-γ或白细胞介素（IL）-4刺激分化后的巨噬细胞。IL-4可增强唾液酸结合免疫球蛋白样凝集素-10（Siglec-10）的表达，而LPS加IFN-γ可降低Siglec-7的表达。Siglec-9的表达不受这些刺激的影响。敲低Siglec-9可增强LPS或LPS加IFN-γ刺激诱导的CC趋化因子受体7（CCR7）的表达，并降低IL-4诱导的CD200受体（CD200R）的表达。这些结果表明，Siglec-9是人类血液单核细胞/巨噬细胞中的主要Siglecs之一，并调节先天免疫。
