Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran; Department of Materials Science and Nanotechnology Engineering, Faculty of Engineering, Near East University, 99138 Nicosia, North Cyprus, Mersin 10, Turkey.
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Dec 5;205:614-621. doi: 10.1016/j.saa.2018.07.088. Epub 2018 Jul 31.
Herein, development of a reliable and specific fluorometric assay was disclosed for the sensitive detection of atropine. The method was designed using the surface molecularly imprinted polymer on high fluorescent graphene quantum dots (GQDs). Molecularly imprinted polymer capped GQDs (MIP-GQDs) were prepared through the common co-polymerization reaction of 3-(3-aminopropyl) triethoxysilane (APTES) and tetraethyl orthosilicate (TEOS), act as the main functional and cross-linking monomers, respectively. The used template for this reaction was atropine. The created blue luminescent MIP-GQDs composite, which had a great affinity to adsorb atropine from the sample solution, could lead to a notable fluorescence quenching. In fact, GQDs act as the recognizing antenna for adsorbed atropine into the specific MIP sites. The linear association between the observed quenching effect and atropine concentration was exploited to design a selective assay to the detection of atropine. After optimization process, a linear calibration graph was achieved in the atropine concentration range of 0.5-300 ng mL with a detection limit of 0.22 ng mL. Exploitation of high specific MIP technique along with high fluorescent GQDs provided a highly selective and sensitive assay for atropine as a model analyte. It was adequately utilized for the analysis of atropine in biological samples.
本文介绍了一种可靠且特异的荧光分析法，用于灵敏检测阿托品。该方法采用表面分子印迹聚合物（MIP）修饰在高荧光石墨烯量子点（GQDs）上设计而成。分子印迹聚合物（MIP）修饰的 GQDs（MIP-GQDs）是通过 3-（3-氨丙基）三乙氧基硅烷（APTES）和正硅酸乙酯（TEOS）的共聚合反应制备的，分别作为主要功能单体和交联单体。该反应的模板是阿托品。所制备的蓝色发光 MIP-GQDs 复合材料对从样品溶液中吸附的阿托品具有很强的亲和力，导致明显的荧光猝灭。实际上，GQDs 作为识别天线，将吸附的阿托品带入特定的 MIP 位点。利用观察到的猝灭效应与阿托品浓度之间的线性关系，设计了一种选择性分析方法来检测阿托品。经过优化过程，在阿托品浓度范围为 0.5-300ng/mL 时，获得了线性校准曲线，检测限为 0.22ng/mL。高特异性 MIP 技术与高荧光 GQDs 的结合为阿托品作为模型分析物提供了一种高选择性和高灵敏度的分析方法。它被充分用于生物样品中阿托品的分析。
