College of Veterinary Medicine , Nanjing Agricultural University , Nanjing 210095 , P. R. China.
J Agric Food Chem. 2018 Aug 29;66(34):8999-9009. doi: 10.1021/acs.jafc.8b02732. Epub 2018 Aug 20.
The aim of this study is to explore the impact of sodium butyrate on d-glutamyl- meso-diaminopimelic acid (iE-DAP)-induced liver inflammation in dairy goats during subacute ruminal acidosis (SARA) caused by high-concentrate feed. To achieve this aim, 12 lactating dairy goats were randomly divided into two groups: a high-concentrate feed group ( n = 6, concentrate/forage = 6:4) as the control group and a sodium butyrate (SB) with high-concentrate feed group ( n = 6, concentrate/forage = 6:4, with 1% SB by wt.) as the treatment group. A rumen pH below 5.6 lasted for at least 4 h/d due to long-term HC feeding. The concentration of iE-DAP was significantly lower (11.67 ± 3.85 μg/mL, and 7.74 ± 1.46 μg/mL, at the fourth h and sixth h of feeding, respectively) in the SB-treated group than that in the HC group (51.45 ± 5.71 μg/mL, and 18.31 ± 3.83 μg/mL, at the fourth h and sixth h of feeding, respectively). Meanwhile, SB significantly suppressed the mRNA expression of inflammatory genes (NOD1, RIPK2, TAK1, NF-κB/p65, ERK, JNK2, p38, IL-1β, TNF-α, CCL5, CCL20, CXCL12, FOS, β-defensin/LAP). Moreover, the protein expression of NOD1, p-IκBα, p-NF-κB/p-p65, p-ERK1/2, p-JNK, p-p38, and HDAC3 was significantly downregulated in the HC+SB group. In conclusion, iE-DAP-induced inflammation and liver disruption generated by the HC diet was mitigated by SB treatment.
本研究旨在探讨丁酸钠（SB）对高精料饲粮诱导的亚急性瘤胃酸中毒（SARA）条件下奶山羊 d-谷氨酸- meso-二氨基庚二酸（iE-DAP）诱导的肝炎症的影响。选择 12 只泌乳奶山羊，随机分为两组：高精料饲粮组（ n = 6，精粗比为 6:4）作为对照组，高精料饲粮+SB 组（ n = 6，精粗比为 6:4，饲粮中添加 1% SB 以干物质计）作为处理组。由于长期高精料饲喂，山羊瘤胃 pH 持续低于 5.6 至少 4 h/d。在 SB 处理组中，iE-DAP 的浓度在饲喂第 4 小时和第 6 小时时分别显著低于 HC 组（分别为 11.67 ± 3.85 μg/mL 和 7.74 ± 1.46 μg/mL）（分别为 51.45 ± 5.71 μg/mL 和 18.31 ± 3.83 μg/mL）。同时，SB 显著抑制了炎症基因（NOD1、RIPK2、TAK1、NF-κB/p65、ERK、JNK2、p38、IL-1β、TNF-α、CCL5、CCL20、CXCL12、FOS、β-防御素/LAP）的 mRNA 表达。此外，在 HC+SB 组中，NOD1、p-IκBα、p-NF-κB/p-p65、p-ERK1/2、p-JNK、p-p38 和 HDAC3 的蛋白表达均显著下调。综上所述，SB 处理减轻了高精料饲粮诱导的 iE-DAP 诱导的炎症和肝损伤。
