Circular Agriculture Research Center , Jiangsu Academy of Agricultural Sciences , Nanjing , Jiangsu 210014 , People's Republic of China.
Key Laboratory of Crop and Livestock Integrated Farming , Ministry of Agriculture , Nanjing , Jiangsu 210014 , People's Republic of China.
J Agric Food Chem. 2018 Aug 22;66(33):8729-8736. doi: 10.1021/acs.jafc.8b03108. Epub 2018 Aug 9.
We investigated the effect of sodium butyrate feeding on the disruption of ruminal epithelium barrier function in goats fed a high-concentrate diet. A total of 18 male Boer goats (live weight of 31.75 ± 1.35 kg, aged 1 year) were randomly assigned to three groups, which were fed a low-concentrate diet (LC), a high-concentrate diet (HC), or a high-concentrate diet with 1% sodium butyrate by weight (SH) for 9 weeks. We found that the pH of rumen fluid in the SH and LC groups was higher than that in the HC group. The activity of protein kinase C (PKC) kinase in the rumen epithelium was higher in the HC group than that in the LC and SH groups. The mRNA expression and phosphorylated protein levels of mitogen-activated protein kinases (MAPKs) in the rumen epithelium were lower in the SH and LC groups than those in the HC group. The DNA methylation rate of occludin was higher in the HC group than that in the SH and LC groups. The mRNA and protein expression of claudin-1, claudin-4, occludin, and zona occludin-1 was greater in the SH and LC groups than that in the HC group. In addition, sodium butyrate mitigated damage to the rumen epithelium caused by the HC diet. Together, our results suggest that the supply of sodium butyrate reverses the damage of rumen epithelium tight junction by inhibiting PKC and MAPK signaling pathways and is protective to the rumen epithelium during subacute rumen acidosis.
我们研究了丁酸钠喂养对高浓度日粮喂养山羊瘤胃上皮屏障功能破坏的影响。将 18 只雄性布尔山羊（活重 31.75±1.35kg，年龄 1 岁）随机分为三组，分别饲喂低浓度日粮（LC）、高浓度日粮（HC）或高浓度日粮加 1%丁酸钠（SH），共 9 周。我们发现 SH 和 LC 组瘤胃液 pH 值高于 HC 组。HC 组瘤胃上皮蛋白激酶 C（PKC）激酶活性高于 LC 和 SH 组。SH 和 LC 组瘤胃上皮丝裂原活化蛋白激酶（MAPKs）mRNA 表达和磷酸化蛋白水平低于 HC 组。occludin 的 DNA 甲基化率在 HC 组高于 SH 和 LC 组。SH 和 LC 组的 claudin-1、claudin-4、occludin 和 zona occludin-1 的 mRNA 和蛋白表达均高于 HC 组。此外，丁酸钠缓解了 HC 日粮引起的瘤胃上皮损伤。总之，我们的研究结果表明，丁酸钠的供应通过抑制 PKC 和 MAPK 信号通路逆转了高浓度日粮引起的瘤胃上皮紧密连接的损伤，在亚急性瘤胃酸中毒期间对瘤胃上皮具有保护作用。
