Dhurandhar D, Bharihoke V, Kalra S
Department of Anatomy, University College of Medical Sciences, Dilshad Garden, Delhi, India.
Department of Anatomy, University College of Medical Sciences, Dilshad Garden, Delhi, India.
Morphologie. 2018 Sep;102(338):197-204. doi: 10.1016/j.morpho.2018.07.003. Epub 2018 Aug 2.
Sucralose is an artificial sweetener freely available under different brand names over the counter. This study is aimed at evaluating the histopathological changes in the liver after administration of sub lethal dose of pure sucralose.
Inbred adult Wistar albino rats weighing about 150-200g of either sex, were divided into 6 control rats and 6 experimental rats. Experimental rats were given sucralose orally by gavage in the dose of 3g/kg/day dissolved in distilled water for 30 days whereas Control rats received equal quantity of distilled water by the same route. The animals were anesthetized with anesthetic ether and then perfused with 10% formal saline. Livers were dissected out. Pieces each having thickness 5mm were taken for paraffin sectioning. 5 micron thick sections were cut using a rotary microtome. Hundred slides were made from each liver and stained with hematoxylin and eosin, periodic acid-schiff (PAS) and Masson's trichrome stain. Slides were evaluated for histomorphological changes.
Experimental rats showed features of patchy degeneration of hepatocytes along with Kupffer cells hyperplasia, lymphocytic infiltration, sinusoidal dilatation and fibrosis indicating a definite hepatic damage on regular ingestion of sucralose. Sinusoidal width was also found to be increased in experimental animals as compared to controls.
It is suggestive that sucralose should be taken with caution to avoid hepatic damage. Effects of ingestion of sucralose on human subjects should be investigated further.
三氯蔗糖是一种可在柜台以不同品牌名自由购买的人工甜味剂。本研究旨在评估给予亚致死剂量纯三氯蔗糖后肝脏的组织病理学变化。
体重约150 - 200克的近交成年Wistar白化大鼠，雌雄不限，分为6只对照大鼠和6只实验大鼠。实验大鼠经口灌胃给予溶解于蒸馏水中的三氯蔗糖，剂量为3克/千克/天，持续30天，而对照大鼠经相同途径给予等量蒸馏水。动物用麻醉乙醚麻醉，然后用10%的甲醛生理盐水灌注。取出肝脏。取厚度为5毫米的切片进行石蜡切片。使用旋转切片机切成5微米厚的切片。从每个肝脏制作100张玻片，并用苏木精和伊红、过碘酸 - 希夫（PAS）和马森三色染色法染色。评估玻片的组织形态学变化。
实验大鼠表现出肝细胞局灶性变性的特征，伴有库普弗细胞增生、淋巴细胞浸润、窦状隙扩张和纤维化，表明长期摄入三氯蔗糖会导致明确的肝损伤。与对照组相比，实验动物的窦状隙宽度也增加。
提示应谨慎使用三氯蔗糖以避免肝损伤。应进一步研究三氯蔗糖对人类受试者的影响。
