Haq Nadia, Tafweez Raafea, Saqib Sadia, Bokhari Zahra Haider, Ali Irfan, Syami Ahmed Fawad
Department of Anatomy, King Edward Medical University, Lahore, Pakistan.
Department of Physiotherapy, Mayo Hospital, Lahore, Pakistan.
J Coll Physicians Surg Pak. 2019 Sep;29(9):848-851. doi: 10.29271/jcpsp.2019.09.848.
To determine the cytoplasmic fatty change of rat liver due to artificial sweeteners - aspartame and sucralose.
Experimental study.
Department of Anatomy, King Edward Medical University, Lahore, in collaboration with the University of Veterinary and Animal Sciences Lahore, from May to October 2016.
Adult Wistar albino rats were randomly divided into five groups. Group I was control group (animals were given drinking water by oral gavage). The animals of group II and III were given low (40 mg/kg body weight) and high dose (1000 mg/kg body weight) of aspartame, respectively. Animals of group IV and V were given low (5 mg/kg body weight) and high dose (1000 mg/kg body weight) of sucralose respectively by oral gavage. Doses were given once daily, six days a week for a total duration of 8 weeks. At the end of experiment, livers of all animal groups were observed for fatty change of cytoplasm.
Artificial sweeteners cause cytoplasmic fatty change of varying degrees. This change was more marked in high dose group of aspartame (group III) as compared to low dose group (group II). In sucralose group, this change was seen only in high dose group (group V).
Both artificial sweeteners-induced fatty changes in rat liver; the effect was least pronounced with low dose of sucralose.
确定人工甜味剂阿斯巴甜和三氯蔗糖是否会导致大鼠肝脏细胞质脂肪变性。
实验研究。
拉合尔爱德华国王医科大学解剖学系与拉合尔兽医与动物科学大学合作，于2016年5月至10月进行。
将成年Wistar白化大鼠随机分为五组。第一组为对照组（通过口服灌胃给予动物饮用水）。第二组和第三组动物分别给予低剂量（40毫克/千克体重）和高剂量（1000毫克/千克体重）的阿斯巴甜。第四组和第五组动物分别通过口服灌胃给予低剂量（5毫克/千克体重）和高剂量（1000毫克/千克体重）的三氯蔗糖。每天给药一次，每周六天，共持续8周。实验结束时，观察所有动物组肝脏细胞质的脂肪变性情况。
人工甜味剂会导致不同程度的细胞质脂肪变性。与低剂量组（第二组）相比，阿斯巴甜高剂量组（第三组）的这种变化更为明显。在三氯蔗糖组中，这种变化仅在高剂量组（第五组）中出现。
两种人工甜味剂均会引起大鼠肝脏脂肪变性；低剂量三氯蔗糖的影响最不明显。
