Chen Junwen, Wu Wei, Zheng Youliang, Hou Kai, Xu Yingwen, Zai Juanyuan
Agronomy College, Sichuan Agricultural University, Ya'an 625014, China.
Zhongguo Zhong Yao Za Zhi. 2010 Jan;35(2):149-53. doi: 10.4268/cjcmm20100205.
To study the optimum conditions of simulated drought stress, and screen the indexes of drought resistance and comprehensively assess the drought resistance of the Angelica dahurica resources during seedling stage.
Investigations were carried out on the changes of height, root length, root-shoot ration, contents of soluble sugar, proline and malondialdehyde under polyethylene glycol (PEG-6000)-simulated drought stress. A comprehensive evaluation on the drought resistance of different (varietal) species of A. dahurica during seedling stage was applied by using the method of subordinate function. And the drought resistance indexes were selected out by applying the method of grey correlative degree analysis.
Drought stress of 9 days with 20% PEG was the optimum condition for the simulation of drought stress. The results showed that the drought resistant capability decreased in the order as follows, A. dahurica from Sichuan province, A. dahurica from Henan province, A. dahurica from Hebei province and A. dahurica from Zhejiang province. And the order of correlative degree of drought resistance and indexes was: soluble sugar > root length > proline > root-shoot ration > total content of chlorophyll > chlorophyll b > chlorophyll a > height > malondialdehyde.
Osmotic adjustment substance and the indexes related to the root have more influence on the drought resistance of A. dahurica during seedling stage. A. dahurica from Sichuan province shows the highest drought resistance during seedling stage.
研究模拟干旱胁迫的最佳条件，筛选白芷资源苗期抗旱指标并综合评价其抗旱性。
对聚乙二醇（PEG - 6000）模拟干旱胁迫下白芷的株高、根长、根冠比、可溶性糖、脯氨酸和丙二醛含量变化进行研究。采用隶属函数法对不同（变种）白芷苗期抗旱性进行综合评价，应用灰色关联度分析法筛选抗旱指标。
20% PEG处理9天为模拟干旱胁迫的最佳条件。结果表明，抗旱能力由强到弱依次为：四川白芷、河南白芷、河北白芷、浙江白芷。抗旱性与指标的关联度顺序为：可溶性糖＞根长＞脯氨酸＞根冠比＞叶绿素总量＞叶绿素b＞叶绿素a＞株高＞丙二醛。
渗透调节物质及与根系相关的指标对白芷苗期抗旱性影响较大。四川白芷苗期抗旱性最强。
