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盘基网柄菌核糖体DNA染色质的甲磺酰丙基-乙二胺四乙酸-铁（II）切割

Methidiumpropyl-EDTA-iron(II) cleavage of ribosomal DNA chromatin from Dictyostelium discoideum.

作者信息

Parish R W, Banz E, Ness P J

出版信息

Nucleic Acids Res. 1986 Mar 11;14(5):2089-107. doi: 10.1093/nar/14.5.2089.

DOI:10.1093/nar/14.5.2089
PMID:3008086
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339645/
Abstract

We have used methidiumpropyl-EDTA-iron(II) [MPE.Fe(II)] in parallel with micrococcal nuclease to investigate the chromatin structure of the extrachromosomal palindrome ribosomal RNA genes of Dictyostelium. Confirming our earlier results with micrococcal nuclease (1,2), MPE.Fe(II) digested the coding region of rapidly transcribing rRNA genes as a smear, indicating the absence or severe disruption of nucleosomes, whereas in slowly transcribing rRNA genes, a nucleosomal ladder was produced. In the central non-transcribed spacer region of the palindrome, MPE.Fe(II) digestion resulted in a normal nucleosomal repeat, whereas micrococcal nuclease gave a complex banding pattern. The difference is attributed to the lower sequence specificity of MPE.Fe(II) compared to micrococcal nuclease. In the terminal region of the palindrome, however, both substances gave a complex chromatin digestion pattern. In this region the DNA appears to be packaged in structures strongly positioned with respect to the underlying DNA sequence.

摘要

我们将甲鎓丙基 - EDTA - 铁（II）[MPE.Fe(II)]与微球菌核酸酶同时使用，以研究盘基网柄菌染色体外回文核糖体RNA基因的染色质结构。与我们早期使用微球菌核酸酶的结果（1,2）一致，MPE.Fe(II) 将快速转录的rRNA基因的编码区域消化成一片模糊，表明核小体不存在或严重破坏，而在缓慢转录的rRNA基因中，则产生了核小体梯带。在回文的中央非转录间隔区，MPE.Fe(II) 消化产生正常的核小体重复，而微球菌核酸酶则产生复杂的条带模式。这种差异归因于与微球菌核酸酶相比，MPE.Fe(II) 的序列特异性较低。然而，在回文的末端区域，两种物质都产生了复杂的染色质消化模式。在该区域，DNA似乎被包装在相对于基础DNA序列强烈定位的结构中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/c75ed8d6e838/nar00274-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/37bc214ed4a8/nar00274-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/94c75abdc0d0/nar00274-0166-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/9df4f0eb169b/nar00274-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/1ef760cbd870/nar00274-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/54b40c2b593a/nar00274-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/9eb7232048b3/nar00274-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/e876f7c8e1f4/nar00274-0171-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/b17568290e79/nar00274-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/c75ed8d6e838/nar00274-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/37bc214ed4a8/nar00274-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/94c75abdc0d0/nar00274-0166-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/9df4f0eb169b/nar00274-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/1ef760cbd870/nar00274-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/54b40c2b593a/nar00274-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/9eb7232048b3/nar00274-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/e876f7c8e1f4/nar00274-0171-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/b17568290e79/nar00274-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/339645/c75ed8d6e838/nar00274-0174-a.jpg

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引用本文的文献

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Nucleic Acids Res. 1989 Mar 25;17(6):2333-50. doi: 10.1093/nar/17.6.2333.
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Tandem repeats in extrachromosomal ribosomal DNA of Dictyostelium discoideum, resulting from chromosomal mutations.

本文引用的文献

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Metabolism of major cell components during slime mold morphogenesis.黏菌形态发生过程中主要细胞成分的代谢
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Chromatin structure and gene activity: the role of nonhistone chromosomal proteins.染色质结构与基因活性：非组蛋白染色体蛋白的作用
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A structural concept for nucleoli of Dictyostelium discoideum deduced from dissociation studies.从解离研究推导得出的盘基网柄菌核仁的结构概念。
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