Bukrinskaia A G, Ustina V P, Sharova N K, Kadoshnikov Iu P, Kornilaeva G V
Vopr Virusol. 1986 Jan-Feb;31(1):45-51.
The structure of human parainfluenza type 3 virus was studied by electron microscopy and virion fractionation by treatment with a detergent and high ionic strength. The protein spectrum of the virus was studied. The presence of 6 structural proteins was revealed of which two, HN and F, are glycoproteins. Intracellular cleavage of F0 protein into F1+2 proteins was demonstrated in a pulse-chase experiment. A tighter binding of HN protein than of F protein with the virus lipoprotein membrane was observed which may be useful for obtaining purified F protein preparations.
通过电子显微镜以及用去污剂和高离子强度处理进行病毒粒子分级分离，对人3型副流感病毒的结构进行了研究。研究了该病毒的蛋白质谱。发现该病毒存在6种结构蛋白，其中两种，即HN和F，是糖蛋白。在脉冲追踪实验中证实了F0蛋白在细胞内裂解为F1 + 2蛋白。观察到HN蛋白与病毒脂蛋白膜的结合比F蛋白更紧密，这可能有助于获得纯化的F蛋白制剂。
