Sharova N K, Bukrinskaia A G
Vopr Virusol. 1987 Mar-Apr;32(2):173-5.
Proteins of human parainfluenza type 3 virus propagated in green monkey kidney cultures are described. The presence of 4 major and 3 minor polypeptides is described along with their molecular weights. Protein F1 was found as a double band, its both components having a similar intensity. Only the upper band of F1 protein was found in the virus grown in Vero cell culture which has a low sensitivity for this virus. Fractionation of the virus and analysis of the proteins of subviral structures were carried out. Nucleocapsid had a buoyant density of 1.32 g/cm3 in cesium chloride and contained major NP protein and minor P and L proteins.
描述了在绿猴肾细胞培养物中繁殖的人副流感3型病毒的蛋白质。描述了4种主要和3种次要多肽的存在及其分子量。发现F1蛋白为一条双带,其两个组分强度相似。在对该病毒敏感性较低的Vero细胞培养物中生长的病毒中,仅发现F1蛋白的上带。对病毒进行了分级分离,并分析了亚病毒结构的蛋白质。核衣壳在氯化铯中的浮力密度为1.32 g/cm3,含有主要的NP蛋白以及次要的P蛋白和L蛋白。