Panigrahi P, Mohanty S B, Maheshwari R K, Friedman R M
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland Campus, College Park 20742.
Vet Microbiol. 1987 Mar;13(3):205-10. doi: 10.1016/0378-1135(87)90083-6.
Six structural proteins of bovine parainfluenza-3 virus (PI-3V) labeled with [35S]-methionine could be resolved by polyacrylamide gel electrophoresis (PAGE). Five structural proteins of this virus had been previously reported. The 6 proteins found in this study were: L, a 180,000 (180 kD) molecular weight (MW) large protein; P, 83 kD phosphoprotein; HN, 69 kD hemagglutinin-neuraminidase glycoprotein; NP, 66 kD nucleocapsid protein; F, 55 kD fusion glycoprotein; and M, 38 kD matrix protein. Selective labeling with [2-3H]-mannose revealed only HN and F glycoprotein bands. A cellular actin protein (43 kD), associated with many enveloped viruses, was also found as a seventh protein in bovine PI-3V.
用[35S] - 甲硫氨酸标记的牛副流感3病毒（PI - 3V）的六种结构蛋白可通过聚丙烯酰胺凝胶电泳（PAGE）分离。该病毒的五种结构蛋白此前已有报道。本研究中发现的6种蛋白质分别是：L，一种分子量（MW）为180,000（180 kD）的大蛋白；P，83 kD磷蛋白；HN，69 kD血凝素 - 神经氨酸酶糖蛋白；NP，66 kD核衣壳蛋白；F，55 kD融合糖蛋白；以及M，38 kD基质蛋白。用[2 - 3H] - 甘露糖进行选择性标记仅显示出HN和F糖蛋白条带。一种与许多包膜病毒相关的细胞肌动蛋白（43 kD）也作为牛PI - 3V中的第七种蛋白被发现。
