Goffinet A M, Caviness V S
Brain Res. 1986 Feb 26;366(1-2):193-202. doi: 10.1016/0006-8993(86)91295-3.
The distribution of alpha-1 and beta-1 adrenoceptors has been studied in the midbrain and forebrain of normal and reeler mutant mice, using autoradiographic visualization of radioiodinated HEAT and ICYP, respectively. All cortical structures and nuclear groups of the murine forebrain and midbrain bind ICYP and HEAT. For each ligand, there is substantial regional variation in binding density and these variations tend to observe boundaries between nuclei or cortical regions or the stratification of cortical regions. Regional variations in binding densities are generally different for ICYP and HEAT. Binding sites for ICYP are distributed densely throughout all fields of the neocortex (particularly, layers I-III greater than VI) and paleocortex, the striatum, pallidum, substantia nigra and superficial strata of the superior colliculus. Dense concentrations of binding sites for HEAT in cortical structures, by contrast, are limited to frontal (all layers except IV) and anterior cingulate regions of the neocortex and, as with ICYP, the stratum lacunosum-moleculaire of the regio superior of the hippocampal formation. In subcortical structures, again in contrast to the pattern with ICYP, binding density is greatest in the principal nuclei of the dorsal thalamus and the septal nuclei. The regional binding patterns of both ICYP and HEAT in the reeler brain are identical to those in the normal animal. Differential laminar binding patterns within the neocortex are approximately inverted in the two genotypes, however. Thus, binding of ICYP is densest in an inner zone of the mutant, but in the outer 3 layers of the normal neocortex. Binding of this ligand is of relatively lower density in an outer zone of the mutant and in the inner 3 layers of the normal neocortex. Similar inversions are characteristic of the laminar binding patterns of HEAT in the frontal, primary sensory and associational cortical regions of the two genotypes where densest binding is encountered superficially in reeler but at deeper levels of the normal neocortex.
分别使用放射性碘化HEAT和ICYP的放射自显影可视化技术，研究了正常小鼠和reeler突变小鼠中脑和前脑中α-1和β-1肾上腺素能受体的分布。小鼠前脑和中脑的所有皮质结构和核团均与ICYP和HEAT结合。对于每种配体，结合密度存在显著的区域差异，这些差异往往出现在核或皮质区域之间的边界处，或皮质区域的分层处。ICYP和HEAT的结合密度区域差异通常不同。ICYP的结合位点密集分布于新皮质的所有区域（特别是I-III层大于VI层）、旧皮质、纹状体、苍白球、黑质和上丘的浅层。相比之下，皮质结构中HEAT结合位点的密集浓度仅限于新皮质的额叶（除IV层外的所有层）和前扣带区域，与ICYP一样，海马结构上区的分子层隙状层也有。在皮质下结构中，与ICYP的模式再次不同，背侧丘脑的主要核团和隔核中的结合密度最大。reeler脑内ICYP和HEAT的区域结合模式与正常动物相同。然而，两种基因型的新皮质内不同的层状结合模式大致相反。因此，ICYP在突变体的内层结合最密集，但在正常新皮质的外三层。该配体在突变体的外层区域和正常新皮质的内三层结合密度相对较低。类似的反转是两种基因型额叶皮质、初级感觉皮质和联合皮质区域HEAT层状结合模式的特征，其中reeler在表面结合最密集，而在正常新皮质的较深层。
