用一系列二甲磺酸酯处理后组蛋白与DNA之间的交联作用。
Cross-linking between histones and DNA following treatment with a series of dimethane sulphonate esters.
作者信息
Hartley J A, Fox B W
出版信息
Cancer Chemother Pharmacol. 1986;17(1):56-62. doi: 10.1007/BF00299867.
The cross-linking of the nucleohistone to the associated DNA by a series of methanesulphonates of the busulphan series (CH3SO2O(CH2)nOSO2CH3) from n = 4-9 has been studied by gel electrophoresis, by the use of 35S labelling and by pyrenemaleimide competition. It has been shown that all the dimethanesulphonates react with the Cys 114 of histone H3, and that octamethylene dimethanesulphonate (n = 8) cross-links histone H3 with DNA via the sulphydryl group, as well as forming H3-H3 dimers.
通过凝胶电泳、使用35S标记以及芘马来酰亚胺竞争法，研究了一系列白消安系列的甲磺酸盐（CH3SO2O(CH2)nOSO2CH3，n = 4 - 9）使核组蛋白与相关DNA交联的情况。结果表明，所有二甲磺酸盐都与组蛋白H3的Cys 114发生反应，并且八亚甲基二甲磺酸盐（n = 8）通过巯基使组蛋白H3与DNA交联，同时还形成H3 - H3二聚体。
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