利用合成生物学方法在蓝细菌中可持续生产对香豆酸及其衍生物
Synthetic Biology Approaches to the Sustainable Production of p-Coumaric Acid and Its Derivatives in Cyanobacteria.
作者信息
Xue Yong, He Qingfang
机构信息
Division of Microbiology, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, USA.
Department of Biology, University of Arkansas at Little Rock, Little Rock, AR, USA.
出版信息
Adv Exp Med Biol. 2018;1080:261-277. doi: 10.1007/978-981-13-0854-3_11.
The photosynthetic cyanobacteria are promising candidates for the sustainable production of a plethora of plant secondary metabolites, which are beneficial to human health but are difficult to produce and purify in other systems. This chapter focuses on genetic engineering of Synechocystis PCC 6803 for production of p-coumaric acid and its derivatives. Cyanobacterial engineering approaches are briefly reviewed. Strategies to increase production yield are discussed, including codon optimization of genes expressing enzymatic proteins and a laccase-coding gene knockout from Synechocystis genome which degrades polyphenols.
光合蓝细菌是可持续生产大量植物次生代谢产物的理想候选者，这些次生代谢产物对人类健康有益，但在其他系统中难以生产和纯化。本章重点介绍聚球藻PCC 6803用于生产对香豆酸及其衍生物的基因工程。简要回顾了蓝细菌工程方法。讨论了提高产量的策略，包括对表达酶蛋白的基因进行密码子优化以及敲除聚球藻基因组中降解多酚的漆酶编码基因。
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