Departamento de Ciencias de la Salud, Universidad de Sonora, Campus Cajeme. Blvd. Bordo Nuevo s/n, Antiguo Providencia, Apdo. Postal 85040, Cd. Obregon, Sonora, Mexico.
Departamento de Nutricion Publica y Salud, Coordinacion de Nutricion, Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera al Ejido la Victoria Km 0.6, Apdo. Postal 83304, Hermosillo, Sonora, Mexico.
Curr Top Med Chem. 2018;18(14):1261-1268. doi: 10.2174/1568026618666180810141455.
Recently, Pectin (PEC) and Aloe-Gel (AG) have received great attention for their use in the encapsulation of hydrophobic bioactive compounds such as Carvacrol (CAR). The aim of this study is to assess the physical, chemical and biological properties of a novel PEC/AG film and evaluate its capability to entrap CAR into microencapsulates. For this purpose, the casting method was used to prepare the PEC/AG membranes (70:30 % w/w). The CAR-loaded PEC/AG film was prepared adding different proportions of CAR (0.25%, 0.50% and 1.00% v/v) to the mixture of PEC/AG, previously emulsified with tween 80 (1.0%). The optical properties, Water Vapor Permeability (WVP), ATR-FTIR spectroscopy, microstructure, antibacterial activity and size of microcapsules were evaluated. The PEC/AG membranes loaded with CAR showed yellowish appearance and they were transparent to the UV electromagnetic radiation (190, 200 and 280 nm). The film prepared with the lowest amount of CAR (PC/AG-CAR-0.25%) showed the highest values of WVP (66.2%) and, according to SEM micrograph, the largest microcapsules (≈1005± 39 µm3). The FTIR analysis showed the characteristic absorption peaks located at 1015 cm-1 to 1030 cm-1 and a small shoulder to 990 cm-1 of benzene ring 1:2:4 substituted that suggested the presence of CAR-loaded in the PC/AG film. On the other hand, E. coli O157:H7 showed the highest sensitivity to the PEC/AG-CAR-1.00% film, while S. aureus was not sensitive.
最近，果胶（PEC）和芦荟凝胶（AG）因其在包封疏水性生物活性化合物（如香芹酚（CAR））方面的应用而受到广泛关注。本研究旨在评估新型 PEC/AG 薄膜的物理、化学和生物学特性，并评估其将 CAR 包封入微胶囊的能力。为此，采用浇铸法制备 PEC/AG 膜（70:30%w/w）。通过向 PEC/AG 混合物中添加不同比例的 CAR（0.25%、0.50%和 1.00%v/v）来制备负载 CAR 的 PEC/AG 膜，该混合物之前已用吐温 80（1.0%）乳化。评估了光学性能、水蒸气透过率（WVP）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）、微观结构、抗菌活性和微胶囊的粒径。负载 CAR 的 PEC/AG 膜呈黄色外观，对紫外线电磁辐射（190、200 和 280nm）透明。含有最低量 CAR（PC/AG-CAR-0.25%）的薄膜具有最高的 WVP（66.2%）值，并且根据 SEM 显微照片，其微胶囊最大（≈1005±39µm3）。FTIR 分析显示了位于 1015cm-1 至 1030cm-1 处的特征吸收峰和 990cm-1 处的小肩峰，表明苯环 1:2:4 取代物存在负载 CAR 的 PC/AG 薄膜。另一方面，PEC/AG-CAR-1.00%膜对 E. coli O157:H7 表现出最高的敏感性，而金黄色葡萄球菌则不敏感。
