Cárdenas Diana M, Cucalón Roberto V, Medina-Magües Lex G, Jones Karina, Alemán Rubén A, Alfaro-Núñez Alonzo, Cárdenas Washington B
1 Laboratorio para Investigaciones Biomédicas, Facultad de Ciencias de la Vida, Escuela Superior Politécnica del Litoral, 090920, Guayaquil, Guayas, Ecuador.
2 College of Public Health, Medical and Veterinary Sciences, James Cook University, 4811, Townsville, Queensland, Australia.
J Wildl Dis. 2019 Jan;55(1):169-173. doi: 10.7589/2017-12-295. Epub 2018 Aug 10.
Fibropapillomatosis is a neoplastic disease that afflicts sea turtles. Although it is disseminated worldwide, cases of the disease have not been reported in the southeastern Pacific region. We describe a case of fibropapillomatosis in a green sea turtle ( Chelonia mydas) during its rehabilitation at the Machalilla National Park Rehabilitation Center, Ecuador. Viral presence was confirmed by PCR, targeting fragments of the chelonid alphaherpesvirus 5 (ChHV5) unique long (UL) genes, UL27, UL28, and UL30. The amplicons were sequenced and included in a global phylogenetic analysis of the virus with other reported sequences from GenBank. Results showed that the available viral sequences segregated into five phylogeographic groups: western Atlantic and eastern Caribbean, central Pacific, western Pacific, Atlantic, and eastern Pacific groups. The concatenated ChHV5 sequences from Ecuador clustered with the eastern Pacific sequences.
纤维乳头瘤病是一种折磨海龟的肿瘤性疾病。尽管该病在全球范围内传播，但在东南太平洋地区尚未有病例报告。我们描述了一只绿海龟（蠵龟）在厄瓜多尔马查利拉国家公园康复中心康复期间患纤维乳头瘤病的病例。通过聚合酶链反应（PCR），以龟甲疱疹病毒5（ChHV5）独特长（UL）基因的片段UL27、UL28和UL30为靶点，确认了病毒的存在。对扩增子进行测序，并将其纳入与来自GenBank的其他已报告序列进行的病毒全球系统发育分析中。结果表明，现有的病毒序列分为五个系统地理组：西大西洋和东加勒比、中太平洋、西太平洋、大西洋和东太平洋组。来自厄瓜多尔的串联ChHV5序列与东太平洋序列聚类。
