Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production.

To improve the economic viability of Chlorella as feedstock for food commodities, a serial of concentrations of low-cost sweet sorghum juice (SSJ), alternative to glucose, were used for the fermentation of Chlorella pyrenoidosa. A high biomass and protein production (8.91 g L biomass and 4.52 g L protein) was revealed with 20% SSJ. To further increase productivity, heavy-ion irradiation-mediated mutagenesis was employed to create mutants where a strain K05, with desired phenotypes (increased biomass and protein production in pilot-scale fermentation), was screened. Compared with the parental strain, the production of biomass, proteins, and chlorophylls of mutant K05 increased by 11.6%, 31.8%, and 7.6%, respectively. Production capacities under industrial scale (two-ton) further pinpoint the stability and scalability of mutant K05. These results suggest that advances in cultivation techniques coupled with artificial strain improvement will further promote microalgae as an attractive platform of functional food.