Wu Kangping, Fang Yilin, Hong Biyuan, Cai Yihui, Xie Honglei, Wang Yunpu, Cui Xian, Yu Zhigang, Liu Yuhuan, Ruan Roger, Zhang Qi
State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, College of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Queen Mary School, Jiangxi Medical College, Nanchang University, Nanchang 330047, China.
Foods. 2022 Aug 25;11(17):2579. doi: 10.3390/foods11172579.
High-cost carbon sources are not economical or sustainable for the heterotrophic culture of . In order to reduce the cost, this study used sweet sorghum extract (SE) and its enzymatic hydrolysate (HSE) as alternative carbon sources for the heterotrophic culture of . Under the premise of the same total carbon concentration, the value-added product production performance of cultured in HSE (supplemented with nitrogen sources and minerals) was much better than that in the glucose medium. The conversion rate of the total organic carbon and the utilization rate of the total nitrogen were both improved in the HSE system. The biomass production and productivity using HSE reached 2.51 g/L and 0.42 g/L/d, respectively. The production of proteins and lipids using HSE reached 1.17 and 0.35 g/L, respectively, and the production of chlorophyll-a, carotenoid, and lutein using HSE reached 30.42, 10.99, and 0.88 mg/L, respectively. The medium cost using HSE decreased by 69.61% compared to glucose. This study proves the feasibility and practicability of using HSE as a carbon source for the low-cost heterotrophic culture of .
对于……的异养培养而言，高成本碳源既不经济也不可持续。为了降低成本，本研究使用甜高粱提取物（SE）及其酶解产物（HSE）作为……异养培养的替代碳源。在总碳浓度相同的前提下，在HSE（补充氮源和矿物质）中培养的……的增值产物生产性能远优于在葡萄糖培养基中的性能。HSE系统中总有机碳的转化率和总氮的利用率均有所提高。使用HSE时的生物量产量和生产率分别达到2.51 g/L和0.42 g/L/d。使用HSE时蛋白质和脂质的产量分别达到1.17和0.35 g/L，使用HSE时叶绿素-a、类胡萝卜素和叶黄素的产量分别达到30.42、10.99和0.88 mg/L。与葡萄糖相比，使用HSE的培养基成本降低了69.61%。本研究证明了使用HSE作为……低成本异养培养碳源的可行性和实用性。
