Maiocco Stephanie J, Walker Lindsey M, Elliott Sean J
Department of Chemistry, Boston University, Boston, MA, United States.
Department of Chemistry, Boston University, Boston, MA, United States.
Methods Enzymol. 2018;606:319-339. doi: 10.1016/bs.mie.2018.06.002. Epub 2018 Jul 5.
While protein film electrochemistry (PFE) has proven to be an effective tool in the interrogation of redox cofactors and assessing the electrocatalytic activity of many different enzymes, recently it has been proven to be useful for the study of the redox potentials of the cofactors of AdoMet radical enzymes (AREs). In this chapter, we review the challenges and opportunities of examining the redox cofactors of AREs in a high level of detail, particularly for the deconvolution of redox potentials of multiple cofactors. We comment on how to best assess the electroactive nature of any given ARE, and we see that when applied well, PFE allows for not only determining redox potentials, but also determining proton-coupling and ligand-binding phenomena in the ARE superfamily.
虽然蛋白质膜电化学（PFE）已被证明是研究氧化还原辅因子和评估许多不同酶的电催化活性的有效工具，但最近已证明它对于研究腺苷甲硫氨酸自由基酶（AREs）辅因子的氧化还原电位很有用。在本章中，我们详细回顾了研究AREs氧化还原辅因子的挑战和机遇，特别是对于多个辅因子氧化还原电位的解卷积。我们评论了如何最好地评估任何给定ARE的电活性本质，并且我们发现，当应用得当时，PFE不仅允许确定氧化还原电位，还允许确定ARE超家族中的质子耦合和配体结合现象。
