聚丙烯酰胺凝胶的考马斯亮蓝染色
Coomassie Brilliant Blue Staining of Polyacrylamide Gels.
作者信息
Arndt Claudia, Koristka Stefanie, Feldmann Anja, Bergmann Ralf, Bachmann Michael
机构信息
Institute of Radiopharmaceutical Cancer Research, Radioimmunology, Helmholtz-Zentrum Dresden-Rossendorf e.V. (HZDR), Dresden, Germany.
出版信息
Methods Mol Biol. 2018;1853:27-30. doi: 10.1007/978-1-4939-8745-0_4.
In the past a series of staining procedures for proteins were published. Still, the most commonly used staining dye for proteins is Coomassie Brilliant Blue. The major reason is that Coomassie Brilliant Blue staining is simple, fast, and sensitive. As Coomassie Brilliant Blue is almost insoluble in water a series of procedures including colloidal aqueous procedures has been described.
过去曾发表过一系列蛋白质染色方法。然而，蛋白质最常用的染色染料仍是考马斯亮蓝。主要原因是考马斯亮蓝染色简单、快速且灵敏。由于考马斯亮蓝几乎不溶于水，人们已描述了一系列方法，包括胶体水溶液法。
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