Solignac M, Monnerot M, Mounolou J C
J Mol Evol. 1986;23(1):31-40. doi: 10.1007/BF02100996.
Detailed restriction maps (40 cleavage sites on average) of mitochondrial DNAs (mtDNAs) from the eight species of the melanogaster species subgroup of Drosophila were established. Comparison of the cleavage sites allowed us to build a phylogenetic tree based on the matrix of nucleotide distances and to select the most parsimonious network. The two methods led to similar results, which were compared with those in the literature obtained from nuclear characters. The three chromosomally homosequential species D. simulans, D. mauritiana, and D. sechellia are mitochondrially very related, but exhibit complex phylogenetic relationships. D. melanogaster is their closest relative, and the four species form a monophyletic group (the D. melanogaster complex), which is confirmed by the shared unusual length of their mt genomes (18-19 kb). The other four species of the subgroup (D. yakuba, D. teissieri, D. erecta, and D. orena) are characterized by a much shorter mt genome (16-16.5 kb). The monophyletic character of the D. yakuba complex, however, is questionable. Two species of this complex, D. yakuba and D. teissieri, are mitochondrially indistinguishable (at the level of our investigation) in spite of their noticeable allozymic and chromosomal divergence. Finally, mtDNA distances were compared with the nuclear-DNA distances thus far established. These sequences seem to evolve at rather similar rates, the mtDNA rate being barely double that of nuclear DNA.
构建了果蝇黑腹果蝇物种亚组中八个物种的线粒体DNA（mtDNA）的详细限制图谱（平均40个切割位点）。通过比较切割位点，我们能够基于核苷酸距离矩阵构建系统发育树，并选择最简约的网络。这两种方法得出了相似的结果，并与从核性状获得的文献结果进行了比较。三个染色体同序物种——拟果蝇、毛里求斯果蝇和塞舌尔果蝇，线粒体上非常相关，但呈现出复杂的系统发育关系。黑腹果蝇是它们最近的亲属，这四个物种形成了一个单系群（黑腹果蝇复合体），这一点通过它们mt基因组共有的异常长度（18 - 19 kb）得到了证实。该亚组的其他四个物种（雅库布果蝇、泰斯ieri果蝇、直立果蝇和奥雷纳果蝇）的特征是mt基因组要短得多（16 - 16.5 kb）。然而，雅库布果蝇复合体的单系特征值得怀疑。尽管该复合体的两个物种——雅库布果蝇和泰斯ieri果蝇在等位酶和染色体上存在明显差异，但在我们的研究水平上，它们的线粒体无法区分。最后，将mtDNA距离与目前已确定的核DNA距离进行了比较。这些序列似乎以相当相似的速率进化，mtDNA的速率仅略高于核DNA速率的两倍。
