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从人诱导多能干细胞衍生的视网膜类器官中分离的 CD73 阳性感光细胞的鉴定与移植。

Characterization and Transplantation of CD73-Positive Photoreceptors Isolated from Human iPSC-Derived Retinal Organoids.

机构信息

Institut de la Vision, Sorbonne Université, INSERM, CNRS, 17, Rue Moreau, Paris 75012, France.

INSERM U861, UEVE, CECS, AFM, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases, Corbeil-Essonnes 91100, France.

出版信息

Stem Cell Reports. 2018 Sep 11;11(3):665-680. doi: 10.1016/j.stemcr.2018.07.005. Epub 2018 Aug 9.

DOI:10.1016/j.stemcr.2018.07.005
PMID:30100409
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6135113/
Abstract

Photoreceptor degenerative diseases are a major cause of blindness for which cell replacement is one of the most encouraging strategies. For stem cell-based therapy using human induced pluripotent stem cells (hiPSCs), it is crucial to obtain a homogenous photoreceptor cell population. We confirmed that the cell surface antigen CD73 is exclusively expressed in hiPSC-derived photoreceptors by generating a fluorescent cone rod homeobox (Crx) reporter hiPSC line using CRISPR/Cas9 genome editing. We demonstrated that CD73 targeting by magnetic-activated cell sorting (MACS) is an effective strategy to separate a safe population of transplantable photoreceptors. CD73+ photoreceptor precursors can be isolated in large numbers and transplanted into rat eyes, showing capacity to survive and mature in close proximity to host inner retina of a model of photoreceptor degeneration. These data demonstrate that CD73+ photoreceptor precursors hold great promise for a future safe clinical translation.

摘要

光感受器退行性疾病是导致失明的主要原因之一，而细胞替代是最有前途的策略之一。对于基于干细胞的治疗，使用人诱导多能干细胞（hiPSC），获得同质的光感受器细胞群体至关重要。我们通过使用 CRISPR/Cas9 基因组编辑生成荧光视锥杆同源盒（Crx）报告 hiPSC 系，证实细胞表面抗原 CD73 仅在 hiPSC 衍生的光感受器中表达。我们证明，通过磁激活细胞分选（MACS）靶向 CD73 是分离安全的可移植光感受器群体的有效策略。可以大量分离 CD73+光感受器前体，并将其移植到大鼠眼中，在光感受器退行性病变模型中，显示出在与宿主内视网膜接近的位置存活和成熟的能力。这些数据表明，CD73+光感受器前体具有很大的临床转化为安全治疗的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/a8b90a01d658/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/78bff4b0b76e/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/4950079b6697/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/4d75a32a5b7a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/6794f5a80b3a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/4faf4f2a780b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/244738734868/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/d9d44da50de6/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/a8b90a01d658/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/78bff4b0b76e/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/4950079b6697/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/4d75a32a5b7a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/6794f5a80b3a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/4faf4f2a780b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/244738734868/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/d9d44da50de6/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3b7/6135113/a8b90a01d658/gr7.jpg

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本文引用的文献

1
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Stem Cells. 2018 May;36(5):709-722. doi: 10.1002/stem.2775. Epub 2018 Feb 1.
2
Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner.移植供体或干细胞衍生的视锥细胞都可以在环境依赖的方式下整合并进行物质转移。
Stem Cell Reports. 2018 Feb 13;10(2):406-421. doi: 10.1016/j.stemcr.2017.12.008. Epub 2018 Jan 4.
3
Molecular Anatomy of the Developing Human Retina.
细胞与组织重编程：开启药物研发的新时代。
Pharmacol Rev. 2025 Jun 26;77(5):100077. doi: 10.1016/j.pharmr.2025.100077.
4
Gene and Stem Cell-Based Therapies for Retinal Degenerative Diseases: Update, Challenges, and Future Directions.基于基因和干细胞的视网膜退行性疾病治疗：最新进展、挑战与未来方向
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5
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JCI Insight. 2025 Jun 12;10(14). doi: 10.1172/jci.insight.186338. eCollection 2025 Jul 22.
6
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Int J Mol Sci. 2025 Apr 25;26(9):4078. doi: 10.3390/ijms26094078.
7
Animal models for the evaluation of retinal stem cell therapies.用于评估视网膜干细胞疗法的动物模型。
Prog Retin Eye Res. 2025 May;106:101356. doi: 10.1016/j.preteyeres.2025.101356. Epub 2025 Apr 14.
8
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Prog Retin Eye Res. 2025 Jan;104:101317. doi: 10.1016/j.preteyeres.2024.101317. Epub 2024 Nov 16.
9
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10
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4
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Stem Cells. 2018 Mar;36(3):313-324. doi: 10.1002/stem.2755. Epub 2017 Dec 25.
5
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Stem Cell Reports. 2017 Dec 12;9(6):1898-1915. doi: 10.1016/j.stemcr.2017.10.018. Epub 2017 Nov 16.
6
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Stem Cell Reports. 2017 Sep 12;9(3):820-837. doi: 10.1016/j.stemcr.2017.07.022. Epub 2017 Aug 24.
7
Stem cell therapies for retinal diseases: recapitulating development to replace degenerated cells.用于视网膜疾病的干细胞疗法：重现发育过程以替代退化细胞。
Development. 2017 Apr 15;144(8):1368-1381. doi: 10.1242/dev.133108.
8
Structural divergence of essential triad ribbon synapse proteins among placental mammals - Implications for preclinical trials in photoreceptor transplantation therapy.胎盘哺乳动物中必需三联体带状突触蛋白的结构差异——对光感受器移植治疗临床前试验的启示
Exp Eye Res. 2017 Jun;159:156-167. doi: 10.1016/j.exer.2017.03.005. Epub 2017 Mar 18.
9
Generation of Storable Retinal Organoids and Retinal Pigmented Epithelium from Adherent Human iPS Cells in Xeno-Free and Feeder-Free Conditions.无饲养层和无动物源条件下贴壁人诱导多能干细胞向可储存的视网膜类器官和视网膜色素上皮的生成。
Stem Cells. 2017 May;35(5):1176-1188. doi: 10.1002/stem.2586. Epub 2017 Feb 20.
10
Cone Genesis Tracing by the Chrnb4-EGFP Mouse Line: Evidences of Cellular Material Fusion after Cone Precursor Transplantation.利用Chrnb4-EGFP小鼠品系进行视锥细胞起源追踪：视锥前体细胞移植后细胞物质融合的证据。
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