Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prosp. 33-2, Moscow, 119071, Russia.
School of Engineering, Systems Power & Energy, Glasgow University, Glasgow G12 8QQ, and Holymoor Consultancy Ltd., 360 Ashgate Road, Chesterfield, Derbyshire S40 4BW, UK.
FEMS Microbiol Ecol. 2018 Oct 1;94(10). doi: 10.1093/femsec/fiy152.
We have sequenced metagenome of the microbial community of a deep subsurface thermal aquifer in the Tomsk Region of the Western Siberia, Russia. Our goal was the recovery of near-complete genomes of the community members to enable accurate reconstruction of metabolism and ecological roles of the microbial majority, including previously unstudied lineages. The water, obtained via a 2.6 km deep borehole 1-R, was anoxic, with a slightly alkaline pH, and a temperature around 45°C. Microbial community, as revealed by 16S rRNA gene profiling over 2 years, mostly consisted of sulfate-reducing Firmicutes and Deltaproteobacteria, and uncultured lineages of the phyla Chlorofexi, Ignavibacteriae and Aminicenantes (OP8). 25 composite genomes with more than 90% completeness were recovered from metagenome and used for metabolic reconstruction. Members of uncultured lineages of Chlorofexi and Ignavibacteriae are likely involved in degradation of carbohydrates by fermentation, and are also capable of aerobic and anaerobic respiration. The Chlorofexi bacterium has the Wood-Ljungdahl pathway of CO2 fixation. The recently identified candidate phylum Riflebacteria accounted for 5%-10% of microbial community. Metabolic reconstruction of a member of Riflebacteria predicted that it is an anaerobe capable to grow on carbohydrates by fermentation or dissimilatory Fe(III) reduction.
我们对俄罗斯西伯利亚西部托木斯克地区深部地下热含水层的微生物群落进行了宏基因组测序。我们的目标是恢复群落成员的近完整基因组，以便能够准确重建微生物优势种群的代谢和生态作用，包括以前未研究过的谱系。通过 2.6 公里深的 1-R 钻孔获得的水是缺氧的，呈弱碱性，温度约为 45°C。通过 2 年的 16S rRNA 基因谱分析发现，微生物群落主要由硫酸盐还原菌Firmicutes 和 Deltaproteobacteria 以及未培养的 Chlorofexi、Ignavibacteriae 和 Aminicenantes（OP8）门的谱系组成。从宏基因组中回收了 25 个完整性超过 90%的复合基因组，用于代谢重建。未培养的 Chlorofexi 和 Ignavibacteriae 谱系的成员可能参与碳水化合物的发酵降解，并且还能够进行需氧和厌氧呼吸。Chlorofexi 细菌具有 Wood-Ljungdahl 途径的 CO2 固定。最近发现的候选门 Riflebacteria 占微生物群落的 5%-10%。对 Riflebacteria 成员的代谢重建预测它是一种厌氧菌，能够通过发酵或异化 Fe(III)还原来生长碳水化合物。
