超氧化物介导百草枯对中国仓鼠卵巢细胞的毒性作用。
Superoxide mediates the toxicity of paraquat for Chinese hamster ovary cells.
作者信息
Bagley A C, Krall J, Lynch R E
出版信息
Proc Natl Acad Sci U S A. 1986 May;83(10):3189-93. doi: 10.1073/pnas.83.10.3189.
Abstract
The roles of superoxide and H2O2 in the cytotoxicity of paraquat were assessed in Chinese hamster ovary cells. Neither catalase nor superoxide dismutase inhibited the loss of ability to form colonies when added to the medium. When introduced into the cells, superoxide dismutase but not catalase inhibited the toxicity of paraquat. That superoxide dismutase acted by its known catalytic action is shown by the loss of inhibition when the enzyme was inactivated by H2O2 before being introduced into the cells. The lack of inhibition by catalase, by dimethyl sulfoxide, and by desferoxamine suggests that the toxicity is not mediated by a reaction between H2O2 and superoxide to engender the hydroxyl radical. Exposure of Chinese hamster ovary cells to paraquat may be a suitable means to determine the effects of superoxide anion in cultured cells and the ways in which cells can resist this toxic action.
摘要
在中国仓鼠卵巢细胞中评估了超氧化物和过氧化氢在百草枯细胞毒性中的作用。当将过氧化氢酶和超氧化物歧化酶添加到培养基中时，它们均未抑制形成集落能力的丧失。当将超氧化物歧化酶导入细胞时，它能抑制百草枯的毒性，而过氧化氢酶则不能。当该酶在导入细胞之前被过氧化氢灭活时抑制作用丧失，这表明超氧化物歧化酶是通过其已知的催化作用发挥作用的。过氧化氢酶、二甲基亚砜和去铁胺均未产生抑制作用，这表明毒性不是由过氧化氢与超氧化物之间反应生成羟基自由基所介导的。将中国仓鼠卵巢细胞暴露于百草枯可能是一种合适的方法，用以确定超氧阴离子在培养细胞中的作用以及细胞抵抗这种毒性作用的方式。
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