1- 和 2 个月高剂量 α-亚麻酸处理对健康受试者 C 标记的 α-亚麻酸掺入和转化的影响。

Effect of 1- and 2-Month High-Dose Alpha-Linolenic Acid Treatment on C-Labeled Alpha-Linolenic Acid Incorporation and Conversion in Healthy Subjects.

机构信息

Department of Physiological Chemistry, Faculty of Chemistry, University of Vienna, Austria.

German Research Center of Food Chemistry, Freising, Germany.

出版信息

Mol Nutr Food Res. 2018 Oct;62(20):e1800271. doi: 10.1002/mnfr.201800271. Epub 2018 Sep 19.

SCOPE

The study aims at identifying 1) the most sensitive compartment among plasma phospholipids, erythrocytes, and LDL for studying alpha-linolenic acid (ALA) conversion, and 2) whether ALA incorporation and conversion is saturable after administration of C-labeled ALA-rich linseed oil (LO). The effect of a daily intake of 7 g nonlabeled LO (>43% w/w ALA) for 1 month after bolus administration of 7 g C-labeled LO on day 1, and for 2 months after bolus administration of 7 g C-labeled LO on day 1 and day 29 on C-ALA incorporation and conversion into its higher homologs is investigated in healthy volunteers.

METHODS AND RESULTS

Incorporation and conversion of LO-derived C-labeled ALA is quantified by applying compartmental modeling. After bolus administration, a fractional conversion of approximately 30% from C-ALA to C-DHA is calculated as reflected by the LDL compartment. Treatment with LO for 8 weeks induces a mean reduction of C-ALA conversion to C-DHA by 48% as reflected by the LDL compartment, and a mean reduction of the C-ALA incorporation into LDL by 46%.

CONCLUSION

A 2-month dietary intake of a high dose of LO is sufficient to reach saturation of ALA incorporation into LDL particles, which are responsible for ALA distribution in the body.

研究范围

本研究旨在确定 1）在研究α-亚麻酸（ALA）转化时，血浆磷脂、红细胞和 LDL 中最敏感的部位，以及 2）在给予富含 ALA 的 C 标记亚麻籽油（LO）后，ALA 的掺入和转化是否是饱和的。在给予 7 g C 标记 LO 后的第 1 天和第 1 天和第 29 天给予 7 g C 标记 LO 后的 1 个月内，每天摄入 7 g 非标记 LO（>43% w/w ALA），对健康志愿者中 C-ALA 掺入和转化为其更高同系物的影响进行了研究。

方法和结果

通过应用房室模型来定量 LO 衍生的 C 标记 ALA 的掺入和转化。在给予推注后，LDL 部分计算出约 30%的 C-ALA 转化为 C-DHA 的分数转化率。用 LO 治疗 8 周会导致 LDL 部分反映的 C-ALA 转化为 C-DHA 的平均减少 48%，以及 C-ALA 掺入 LDL 的平均减少 46%。