Department of Physiological Chemistry, Faculty of Chemistry, University of Vienna, Austria.
German Research Center of Food Chemistry, Freising, Germany.
Mol Nutr Food Res. 2018 Oct;62(20):e1800271. doi: 10.1002/mnfr.201800271. Epub 2018 Sep 19.
The study aims at identifying 1) the most sensitive compartment among plasma phospholipids, erythrocytes, and LDL for studying alpha-linolenic acid (ALA) conversion, and 2) whether ALA incorporation and conversion is saturable after administration of C-labeled ALA-rich linseed oil (LO). The effect of a daily intake of 7 g nonlabeled LO (>43% w/w ALA) for 1 month after bolus administration of 7 g C-labeled LO on day 1, and for 2 months after bolus administration of 7 g C-labeled LO on day 1 and day 29 on C-ALA incorporation and conversion into its higher homologs is investigated in healthy volunteers.
Incorporation and conversion of LO-derived C-labeled ALA is quantified by applying compartmental modeling. After bolus administration, a fractional conversion of approximately 30% from C-ALA to C-DHA is calculated as reflected by the LDL compartment. Treatment with LO for 8 weeks induces a mean reduction of C-ALA conversion to C-DHA by 48% as reflected by the LDL compartment, and a mean reduction of the C-ALA incorporation into LDL by 46%.
A 2-month dietary intake of a high dose of LO is sufficient to reach saturation of ALA incorporation into LDL particles, which are responsible for ALA distribution in the body.
本研究旨在确定 1）在研究α-亚麻酸（ALA）转化时，血浆磷脂、红细胞和 LDL 中最敏感的部位，以及 2）在给予富含 ALA 的 C 标记亚麻籽油（LO）后，ALA 的掺入和转化是否是饱和的。在给予 7 g C 标记 LO 后的第 1 天和第 1 天和第 29 天给予 7 g C 标记 LO 后的 1 个月内，每天摄入 7 g 非标记 LO（>43% w/w ALA），对健康志愿者中 C-ALA 掺入和转化为其更高同系物的影响进行了研究。
通过应用房室模型来定量 LO 衍生的 C 标记 ALA 的掺入和转化。在给予推注后，LDL 部分计算出约 30%的 C-ALA 转化为 C-DHA 的分数转化率。用 LO 治疗 8 周会导致 LDL 部分反映的 C-ALA 转化为 C-DHA 的平均减少 48%，以及 C-ALA 掺入 LDL 的平均减少 46%。
高剂量 LO 的 2 个月饮食摄入足以使 ALA 掺入 LDL 颗粒达到饱和，LDL 颗粒负责 ALA 在体内的分布。
