Michel B, Ehrlich S D
Proc Natl Acad Sci U S A. 1986 May;83(10):3386-90. doi: 10.1073/pnas.83.10.3386.
Hybrids composed of phage M13 and plasmid pHV33 were used to study the formation of deletions in Escherichia coli. Eighty to ninety percent of the deletion endpoints were at the position of the nick introduced into the M13 replication origin by the phage gene II protein. This suggests the existence of a novel mechanism of illegitimate recombination.
由噬菌体M13和质粒pHV33组成的杂种用于研究大肠杆菌中缺失的形成。80%至90%的缺失端点位于噬菌体基因II蛋白引入M13复制起点的切口位置。这表明存在一种新的异常重组机制。