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噬菌体M13复制起点处的非法重组。

Illegitimate recombination at the replication origin of bacteriophage M13.

作者信息

Michel B, Ehrlich S D

出版信息

Proc Natl Acad Sci U S A. 1986 May;83(10):3386-90. doi: 10.1073/pnas.83.10.3386.

Abstract

Hybrids composed of phage M13 and plasmid pHV33 were used to study the formation of deletions in Escherichia coli. Eighty to ninety percent of the deletion endpoints were at the position of the nick introduced into the M13 replication origin by the phage gene II protein. This suggests the existence of a novel mechanism of illegitimate recombination.

摘要

由噬菌体M13和质粒pHV33组成的杂种用于研究大肠杆菌中缺失的形成。80%至90%的缺失端点位于噬菌体基因II蛋白引入M13复制起点的切口位置。这表明存在一种新的异常重组机制。

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本文引用的文献

1
Sealing of gaps in duplex DNA by T4 DNA ligase.利用T4 DNA连接酶封闭双链DNA中的缺口。
Nucleic Acids Res. 1982 Mar 11;10(5):1425-37. doi: 10.1093/nar/10.5.1425.
3
Enzymatic synthesis of bacteriophage fd viral DNA.噬菌体fd病毒DNA的酶促合成
Nature. 1982 Apr 29;296(5860):828-32. doi: 10.1038/296828a0.
8
Site-specific recombination in the oriV1 region of the F factor.F因子oriV1区域的位点特异性重组。
Cold Spring Harb Symp Quant Biol. 1984;49:421-34. doi: 10.1101/sqb.1984.049.01.048.

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