Michel B, Ehrlich S D
EMBO J. 1986 Dec 20;5(13):3691-6. doi: 10.1002/j.1460-2075.1986.tb04701.x.
Hybrids between plasmids pC194, pBR322 and the bacteriophage f1 undergo deletions in Escherichia coli. The deletions end most often between nucleotides 1445 and 1446 of pC194. That site probably corresponds to a nick in the replication origin of this plasmid. The localization of the other deletion end appears to be determined by the position of the f1 replication fork. Two models accounting for these data are discussed.
质粒pC194、pBR322与噬菌体f1之间的杂种在大肠杆菌中会发生缺失。缺失大多在pC194的核苷酸1445和1446之间终止。该位点可能对应于该质粒复制起点处的一个切口。另一个缺失末端的定位似乎由f1复制叉的位置决定。文中讨论了两种解释这些数据的模型。
